A marine Pseudomonas species designated WAK-1 strain which was originally isolated from the brown seaweed Undaria pinnatifida produces an acidic muco (A-1)-and a sulfated (B-1) polysaccharides when grown in a seawater medium containing sucrose. Isolation and purification of these polysaccharides have been accomplished by precipitation with ethanol and Cetavlon, followed by DEAE-cellulose column chromatography. These polysaccharide preparations were homogeneous in both ultracentrifugal and electrophoretic analyses. A-1 was composed of galatosamine, glucuronic acid, and pyruvate at a molar ratio of 3:1:1, while B-1 was composed of galactose, glucose, and sulfate at a molar ratios of 2:1:2.
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