Long noncoding RNAs (lncRNAs) are emerging as new players in gene regulation and are associated with the development of cancers. To investigate the important role and mechanism of lncRNAs in the progression of gastric cancer, we screened lncRNAs in gastric cancer tissues and corresponding adjacent tissues, and assessed the effects on gastric cancer. Here, we report that BC032469, a novel lncRNA, expressed highly in gastric cancer tissues, and the upregulation was clinically associated with larger tumor size, poor differentiation and shorter survival of gastric cancer patients. Downregulation of BC032469 resulted in a significant inhibition of proliferation in vitro and in vivo. Mechanistically, BC032469 could directly bind to miR-1207-5p and effectively functioned as a sponge for miR-1207-5p to modulate the derepression of hTERT. Thus, BC032469 may function as a ceRNA to impair miR-1207-5p-dependent hTERT downregulation, suggesting that it may be clinically valuable as a poor prognostic biomarker of gastric cancer.

Using the upgraded Beijing Spectrometer, we have measured the total cross section for e(+)e(-) annihilation into hadronic final states at center-of-mass energies of 2.6, 3.2, 3.4, 3.55, 4.6, and 5.0 GeV. Values of R, sigma(e(+)e(-)-->hadrons)/sigma(e(+)e(-)-->&mgr;(+)&mgr;(-)), are determined.

The appearance of mutations of hepatitis B virus (HBV) Serum hepatitis B virus (HBV) DNA from 4 infants with genome during natural infection can be attributed to the fulminant hepatitis B, 3 infants with acute self-limited hepatioccurrence of errors in its replication via the reverse trantis B, and 15 infants with chronic HBV infection were ampliscription of an RNA intermediate. 1 Various genomic variants fied by polymerase chain reaction followed by direct sequencinvolving the precore or core region of HBV have been deing of the region of HBV genome encoding the major antigenic scribed among patients with fulminant, acute self-limited, as epitopes of hepatitis B surface antigen (HBsAg). All infants well as chronic hepatitis B. 2-4 Hepatitis B surface antigen were born to carrier mothers and administered immunopro-(HBsAg)-specific antibody (anti-HBs) is believed to provide phylaxis from birth. Serum HBV DNA from 13 carrier children protective immunity and aid in clearance of HBV. Point muborn to carrier mothers who did not receive immunoprophytations in the surface (S) gene that result in amino acid laxis and had comparable length of infection were studied as substitutions in the common ''a'' antigenic determinant, controls. An S mutant (residue 126, Thr to Ala) initially found which lies between amino acids 124 and 147, can alter Bin an infant with fulminant hepatitis was replaced by another cell epitopes of HBsAg, leading to immunological escape from S mutant (residue 145, Gly to Arg) 4 days later. In a girl with the host immunity elicited by either vaccination or previous chronic hepatitis B, Ala-126 variant and Arg-145 variant were infection.5 For instance, HBV escape mutants with a glycine found at 17 and 25 months of age, respectively. The Arg-145 (Gly) to arginine (Arg) substitution at amino acid residue variant persisted for 8 years in an asymptomatic male carrier 145 with a loss in the group specific antigen determinant and for 1 year in an infant with chronic hepatitis B. The Ala-were reported to occur in vaccinated Italian infants born to 126 variant persisted for 11 years in one child who had an HBsAg-positive mothers and in liver transplant recipients early loss of hepatitis B e antigen. In the majority of the treated with monoclonal anti-HBs. 6,7 Investigators from Jainfants' mothers, corresponding mutations in HBsAg were not pan, Singapore, and Gambia have described similar vaccine detected in serum by direct sequencing. The S mutants de-escape mutants with nucleotide change resulting in amino tected in three carrier infants were not found in their mothers' acid substitution elsewhere in the ''a'' determinant. [8][9][10] The serum after cloning and sequencing of 10 DNA clones from overall frequency of these mutations is unknown, and their each maternal sample. None of the 13 control patients had biological significance remains speculative. detectable S mutants. These results suggest that S variantsIn Taiwan, a mass immunization program against hepatitis emerge or are selected under the immune pres...

hTERT is the catalytic subunit of the telomerase complex. Elevated expression of hTERT is associated with the expansion and metastasis of gastric tumor. In this study, we aimed to identify novel tumor suppressor miRNAs that restrain hTERT expression. We began our screen for hTERT-targeting miRNAs with a miRNA microarray. miRNA candidates were further filtered by bioinformatic analysis, general expression pattern in different cell lines, gain-of-function effects on hTERT protein and the potential of these effects to suppress hTERT 3′ untranslated region (3′UTR) luciferase activity. The clinical relevance of two miRNAs (miR-1207-5p and miR-1266) was evaluated by real-time RT-PCR. The effects of these miRNAs on cell growth, cell cycle and invasion of gastric cancer cells were measured with CCK-8, flow cytometry and transwell assays. Finally, the ability of these miRNAs to suppress the transplanted tumors was also investigated. Fourteen miRNAs were identified using a combination of bioinformatics and miRNA microarray analysis. Of these fourteen miRNAs, nine were expressed at significantly lower levels in hTERT-positive cell lines compared with hTERT-negative cell lines and five could downregulate hTERT protein expression. Only miR-1207-5p and miR-1266 interacted with the 3′ UTR of hTERT and the expression levels of these two miRNAs were significantly decreased in gastric cancer tissues. These two miRNAs also inhibited gastric tumor growth in vitro and in vivo. Altogether, miR-1207-5p and miR-1266 were determined to be hTERT suppressors in gastric cancer, and the delivery of these two miRNAs represents a novel therapeutic strategy for gastric cancer treatment.

A search for the decays of the Higgs and Z bosons to a ϕ meson and a photon is performed with a pp collision data sample corresponding to an integrated luminosity of 2.7  fb^{-1} collected at sqrt[s]=13  TeV with the ATLAS detector at the LHC. No significant excess of events is observed above the background, and 95% confidence level upper limits on the branching fractions of the Higgs and Z boson decays to ϕγ of 1.4×10^{-3} and 8.3×10^{-6}, respectively, are obtained.