The function of long non-coding RNA LHFPL3 antisense RNA 1 (LHFPL3-AS1) in cancer progression has been studied, while its role in nasopharyngeal carcinoma (NPC) remains unclear. This study aims to unravel the effects of LHFPL3-AS1 on NPC progression via microRNA (miR)-143-5p/homeobox A6 (HOXA6) axis. NPC tissues were collected and NPC cells were cultured. NPC cells were subjected to radiation therapy to construct the radiation therapy resistance NPC cell line. The levels of LHFPL3-AS1, miR-143-5p and HOXA6 in NPC cells and tissues were examined. LHFPL3-AS1, miR-143-5p or HOXA6 expression was changed and then transfected into radiation-resistant NPC cells to detect cell proliferation, colony formation, migration, invasion and cell apoptosis in vitro . The tumorigenesis in nude mice in vivo was conducted to detect tumor growth. The targeting relations among LHFPL3-AS1, miR-143-5p and HOXA6 were validated. It was discovered that LHFPL3-AS1 and HOXA6 expression was elevated while the miR-143-5p level was depleted in radiation-resistant NPC cells and NPC tissues. The silenced LHFPL3-AS1 or augmented miR-143-5p repressed the proliferation, colony formation, migration and invasion of radiation-resistant NPC cells, while accelerated cell apoptosis in vitro . Silenced LHFPL3-AS1 hindered tumor growth in vivo . MiR-143-5p deletion reversed the effects of reduced LHFPL3-AS1; while HOXA6 upregulation reversed the effects of enriched miR-143-5p. LHFPL3-AS1 sponged miR-143-5p that targeted HOXA6. It is concluded that the down-regulated LHFPL3-AS1 retards the development of radiation-resistant NPC cells via sponging miR-143-5p to modulate HOXA6. This study reveals novel therapeutic targets for NPC treatment.

Rapid urban development has changed urban substrate conditions, greatly affecting urban ecology and heating urban environment. Mitigating urban temperature rises by optimizing urban morphology is considered a promising approach; most studies ignore spatial and temporal heterogeneity. This study analyzes how plot spatial form influences urban thermal environment in the main Nanjing area from 2001, 2006, 2011, 2016, and 2021, based on geographically weighted regression models (spatio-temporal- and multi-scale). Results show that: 1. The formation of geothermal heat islands matches the direction of urban expansion, mainly due to changes in land substrate; 2. the spatio-temporal model performs best, indicating that urban morphology and surface thermal environment have obvious spatio-temporal heterogeneity; obvious scale differences exist in each index influencing the heat island effect; and 3. floor area ratio (FAR) and building density (BD) negatively and positively correlate with surface thermal conditions, with gradually increasing effect, respectively. Normalized difference vegetation index (NDVI) and distance from the nearest water body (Dis_W) negatively and positively correlate with surface thermal conditions separately; good ecological infrastructure reduces surface temperatures but shows a gradually weakening effect. Proximity to roads is associated with warmer thermal environment. This study elucidates how urban form influences surface thermal environments and suggests measures to reduce surface temperatures in the main urban Nanjing area.

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Lysine acetylation is a dynamic and reversible modification, which has been proved to be a key posttranslational modification in cellular regulation. However, the low amounts of the acetylated proteins could hardly be detected before enrichment. In this study, for the first time, antibody-immobilized magnetic carbonaceous microspheres were developed for selective enrichment of acetylated proteins and peptides. At first, standard proteins composed of acetylated bovine serum albumin, myoglobin, α-casein and ovalbumin were used as model proteins to verify the enrichment efficiency. Then, the synthesized peptide was employed to confirm the selectivity of the method. Besides, the antibody-immobilized magnetic particles were successfully applied to analyze mouse mitochondrial proteins. After database search, 29 acetylated sites in 26 proteins were identified.