Background Two factors involved in regulation, long noncoding RNA Opa interacting protein 5‐antisense RNA 1 (lncRNA OIP5‐AS1) and microRNA‐147a, were found in cervical cancer. Therefore, the investigation of the specific regulation of miR‐147a by OIP5‐AS1 was performed in cervical cancer. Method The cervical cancer tissues were collected from patients with cervical cancer (n = 50). The expression of OIP5‐AS1, miR‐147a, proteins in epithelial‐mesenchymal transition (EMT) process and insulin‐like growth factor 1 receptor (IGF1R) were measured by quantitative real‐time polymerase chain reaction (qRT‐PCT) or western blotting. Cell motility and the relationship between OIP5‐AS1 and miR‐147a were detected or analyzed by wound healing test, Transwell assay, dual‐luciferase reporter assay, RNA binding protein immunoprecipitation assay or Pearson correlation in OIP5‐AS1, or miR‐147a over‐expressed and/or suppressed cervical cancer cells. Results OIP5‐AS1 showed the high‐expression and miR‐147a showed the low‐expression in tumor tissues collected from patients with cervical cancer and cell lines Hela, CaSki, Siha, and ME‐180. The low‐expression of OIP5‐AS1 suppressed the motility of Caski cells, as well as up‐regulated the level of E‐cadherin, which a key protein in EMT. There were targeting sites between miR‐147a and OIP5‐AS1. OIP5‐AS1 induced the down‐regulation of miR‐147a, so miR‐147a was inversely correlated with OIP5‐AS1. The down‐regulation of miR‐147a increased IGF1R and E‐cadherin, and these increases were alleviated by OIP5‐AS1 knockdown. Conclusion LncRNA OIP5‐AS1 promotes the migration, invasion and EMT of cervical cancer cells via targeting miR‐147a/IGF1R pathway.

A QuEChERS (quick, easy, cheap, effective, rugged, and safe) technique using ultrahigh‐performance liquid chromatography with tandem mass spectrometry for the analysis of spinosad (spinosyn A + spinosyn D), thiocyclam, and nereistoxin in cucumber was developed with mean recoveries of 93−104%, relative standard deviations of ≤9%, and limits of quantification of 0.01 mg/kg. Field trials of spinosad and thiocyclam were performed in 12 representative cultivating areas in China. Field trial results indicate that spinosyn A and spinosyn D easily dissipated in cucumber with half‐lives of 2.48–6.24 and <3 days, respectively. Nereistoxin was produced after thiocyclam application and was more persistent than its parent. The terminal residues of spinosad were all below the maximum residue limits (0.2 mg/kg) in China, whereas the terminal concentration of nereistoxin (calculated as the stoichiometric equivalent of thiocyclam), which was much higher than that of thiocyclam, was far beyond the maximum residue limits of thiocyclam in cucumber (0.01 mg/kg) established by the European Union. The predicted no‐effect concentrations of spinosyn A, spinosyn D, thiocyclam, and nereistoxin leaching into groundwater were estimated using China‐PEARL (Pesticide Emission Assessment at Regional and Local scales) models after application. However, the dietary (food and water) exposure risk quotient for different populations was below 1 with a preharvest interval set at 5 days after the last application, indicating that the application of spinosad and thiocyclam in cucumber was unlikely to pose unacceptable risk for human health. This study provides data for the safe use of spinosad and thiocyclam in cucumber ecosystem.