The influence of temperature and organics on anaerobic ammonium oxidation (ANAMMOX) bacteria activity was investigated in an up-flow ANAMMOX reactor. The kinetics characteristics of cultured ANAMMOX bacteria were analyzed. The results showed that temperature had a significant effect on ANAMMOX bacteria activity, and it was very important to maintain a high temperature to obtain the high activity of ANAMMOX bacteria. There was substrata competition between the denitrification bacteria and ANAMMOX bacteria when organics in the influent. The half saturation constants for ammonium and nitrite of the ANAMMOX bacteria were 151.97 mg•L -1 and 42.598 g•L -1 respectively. The half inhibition constant for ammonium and nitrite of the ANAMMOX bacteria were 555.3 g•L -1 and 200.6 g•L -1 respectively.