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Objective: A simple and sensitive spectrophotometric method has been presented for the determination of phenylephrine hydrochloride by coupling reaction with diazotized sulfacetamide sodium. Methods: The method is based on the diazotization reaction of sulfacetamide sodium with sodium nitrite in the presence of hydrochloric acid to form diazonium salt, which is coupled with the drug in alkaline medium to form azo dye, showing absorption maxima at 425 nm. Results: Calibration plot was linear over the concentration range of 2–24 μg/mL and detection limit of 0.278 μg/mL with a correlation coefficient of 0.9929. All different chemical and physical experimental parameters affecting on the development and stability of the colored product were carefully studied. Conclusions: The proposed method was successfully applied to the determination of phenylephrine in nasal drops with good precision and sensitivity.

INTRODUCTIONProcaine, 4-aminobenzoic acid 2-(diethyl amino) ethyl ester, is a local anesthetic 1 used alone or with penicillin as an antibacterial drug which is marketed as its hydrochloride salt, with molecular weight of 272.8 and the structure is 2 : • HClProcaine and the other local anesthetic drugs prevent the generation and the conduction of the nerve impulses. Their main site of action is the cell membrane, since conduction block can be demonstrated in giant axons from which the axoplasm has been removed. It is used in obstetrics and sometimes for relief pain in the lower back and tooth extraction 3 .Currently, several analytical methods for the quantitation of procaine in pharmaceutical formulations have been reported. Examples of these methods are high performance liquid chromatography 4,5 , gas chromatography 6 , electrophoresis 7,8 , differentialplus voltammetry and electrochemical analysis [9][10][11][12] , chemiluminsense 13 , atomic absorption 14 , ion association titration 15 , flow injection analysis 16,17 , fluorimetry [18][19][20] , sequential injection analysis 21,22 ,liquid chromatography 23 , colorimetry and spectrophotometric methods [24][25][26][27][28][29][30] . However, some of these methods

A simple and highly sensitive cloud point extraction process was suggested for preconcentration of micrograms amount of isoxsuprine hydrochloride (ISX) in pure and pharmaceutical samples. After diazotization coupling of ISX with diazotized sulfadimidine in alkaline medium, the azo-dye product quantitatively extracted into the Triton X-114 rich phase, dissolved in ethanol and determined spectrophotometrically at 490 nm. The suggested reaction was studied with and without extraction and simple comparison between the batch and CPE methods was achieved. Analytical variables including concentrations of reagent, Triton X-114 and base, incubated temperature, and time were carefully studied. Under the selected optimum conditions, the linearity ranges of calibration curves were 1-9 and 0.5-8 µg/mL with detection limits of 0.26 and 0.09 µg/mL of ISX for batch and CPE methods respectively. A relative standard deviation (RSD %) best than 1.98 and 2.67 % with the percentage recoveries range 100.14 and 99.63 % were obtained for both methods respectively. The proposed methods were successfully used in routine analysis of ISX in pharmaceutical forms with high accuracy and reproducibility.