Background Pollen Typhae Carbonisata (PTC) is a type of calcined herb drug that has been used as a hemostatic medicine to promote hemostasis for thousands of years. In this study, we discovered and separated novel water-soluble carbon quantum dots (CQDs, named PTC-CQDs) from aqueous extracts of PTC. These PTC-CDs were characterized using transmission electron microscopy (TEM) and high-resolution TEM, as well as Fourier transform infrared, ultraviolet–visible, and fluorescence spectroscopy. Then, we assessed the anti-hemorrhagic effects and related hemostatic mechanisms of the obtained PTC-CQDs.ResultsThe PTC-CQDs separated from PTC are spherical, monodisperse, and have a narrow size distribution between 2 and 8 nm. In the pharmacology experiment, remarkable anti-hemorrhage effects of PTC-CQDs were revealed. Additionally, the rats showed a profound decrease in activated partial thromboplastin time and increase in fibrinogen and PLT after PTC-CQDs treatment.ConclusionsThese results indicated the explicit hemostasis effect of PTC-CQDs, which not only provided a new idea for the material research of PTC, but have also provided new insights into potential biomedical and healthcare applications of CQDs in the field of haemorrhage control and laid a solid foundation for future drug discovery.

Aim: To explore the hemostatic and hepatoprotective bioactivity of Junci Medulla Carbonisata-derived Carbon Dots (JMC-CDs). Materials & methods: The JMC-CDs were characterized using transmission electron microscopy, HPLC, Fourier transform IR, UV, fluorescence and x-ray photoelectron spectroscopy. The hemostatic effect of JMC-CDs was evaluated and confirmed by trauma hemorrhagic animal models and internal hemorrhage animal model induced by Deinagkistrodon acutus venom. Results: The JMC-CDs ranged in diameter from 1.0 to 8 nm and had a yield of 0.12%. Moreover, JMC-CDs not only possessed remarkable hemostatic efficacy but could also prevent hemorrhage-induced liver injury, as demonstrated by the reduced serum levels of biochemical indicators of liver damage such as aspartate aminotransferase, alanine amino transferase, alkaline phosphatase, total bilirubin and direct bilirubin. Conclusion: The JMC-CDs may have great potentials in clinical practice.

We discovered novel water-soluble carbon dots from aqueous extracts of Cirsium setosum Carbonisata, which have a remarkable hemostatic effect. The study provides evidence to support the further investigation of considerable potential of carbon dots.

Schizonepetae Spica Carbonisata (SSC) has pronounced haemostatic effects for hundreds of years and has been acknowledged in the 2015 Pharmacopoeia of the People's Republic of China (PPRC) as a haemostatic charcoal drug. However, after years of efforts, the underlying mechanism and the material basis is still less defined. In this research, we developed a novel CDs derived from SSC (SSC-CDs) with an average diameter of 1.29-6.87 nm and a quantum yield of 6.31%. SSC was prepared using a modified pyrolysis method and no further modification and external surface passivation agent is required. With abundant surface groups, SSC-CDs showed distinct solubility and bioactivity. In this study, we innovatively used the Deinagkistrodon acutus (D. acutus) venom model as well as the classical haemorrhagic animal model to evaluate the haemostatic bioactivity of SSC-CDs. The results indicated that SSC-CDs had outstanding haemostatic bioactivity and might inhibit the haemorrhagic activity via PLT elevation. According to the results of this study and our previous work, we discovered that CDs derived from different kinds of charcoal drugs presented similarities and differences in the structural feature, physicochemical property and bioactivity. In order to further explore the self-bioactivities, we first named this kind of CDs as "Chinese Medicine charcoal drug nanoparticles" (CMNP). These results may not only provide evidence for further researches of self-bioactivities of CDs but give new insights into potential biomedical and healthcare applications of CDs, therefore, make contributions to future drug discovery.

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