Angelicae Radix (AR) and Ligustici Rhizoma (LR) are both derived from the Umbelliferae plants and contain similar organic acids as their bioactive compounds. Nine of these organic acids, including nicotinic acid, protocatechuic acid, phthalic acid, folinic acid, p-hydroxybenzoic acid, folic acid, vanillic acid, caffeic acid, and ferulic acid were separated by HPLC and CE. Detection at 210 nm with a linear gradient containing 20 mM KH2PO4 (pH 3.5) and H2O-CH3CN in HPLC and with a buffer solution containing 10 mM LTAC, 2 mM Na2HPO4, 9 mM Na2B4O7(pH 9.56), and CH3CN in CE were found to be the most efficient eluents for this separation. The contents of the nine components in crude extracts of either AR or LR could easily be determined within 60 min by LC and within 20 min by CE. The structures of the individual peaks in the LC chromatogram were identified by LC-MS. The effects of buffers on the separation and validation of the two methods were examined.

Determination of moutan tannins by highperformance liquid chromatography and capillary electrophoresisCortex Moutan (Radicis Cortex Moutan), the dried root bark of Paeonia moutan and P. spp., contains a series of water-soluble tannins. With the eight components, 1 4,6-di-O-GG (4,6-di-O-galloyl-D-glucose), 2 1,2,3,6-tetra-O-GG, 3 1,2,3,4,6-penta-O-GG, 4 1,3,4,6-tetra-O-GG, 5 3,4,6-tri-O-GG, 6 1,3,6-tri-O-GG, 7 3,6-di-O-GG, and 8 1,2,6-tri-O-GG, as marker substances, a rapid and efficient method of analysis based on HPLC and CE was developed. Using a phosphate eluent, a 5C18-MS separating column, and a detection wavelength of 280 nm, HPLC was successfully used to analyze the eight constituents within 60 min. The analysis can be completed within 50 min, using the MEKC mode with a buffer composed of borate, SDS, and isopropanol, and a detection wavelength of 210 nm. The detection limit for the marker substances varied from 0.04 to 0.93 lg/mL for the HPLC method and 0.02 to 0.36 lg/mL for the CE method.

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