Plant phenotyping is essential in plant breeding and management. High-throughput data acquisition and automatic phenotypes extraction are common concerns in plant phenotyping. Despite the development of phenotyping platforms and the realization of high-throughput three-dimensional (3D) data acquisition in tall plants, such as maize, handling small-size plants with complex structural features remains a challenge. This study developed a miniaturized shoot phenotyping platform MVS-Pheno V2 focusing on low plant shoots. The platform is an improvement of MVS-Pheno V1 and was developed based on multi-view stereo 3D reconstruction. It has the following four components: Hardware, wireless communication and control, data acquisition system, and data processing system. The hardware sets the rotation on top of the platform, separating plants to be static while rotating. A novel local network was established to realize wireless communication and control; thus, preventing cable twining. The data processing system was developed to calibrate point clouds and extract phenotypes, including plant height, leaf area, projected area, shoot volume, and compactness. This study used three cultivars of wheat shoots at four growth stages to test the performance of the platform. The mean absolute percentage error of point cloud calibration was 0.585%. The squared correlation coefficient R2 was 0.9991, 0.9949, and 0.9693 for plant height, leaf length, and leaf width, respectively. The root mean squared error (RMSE) was 0.6996, 0.4531, and 0.1174 cm for plant height, leaf length, and leaf width. The MVS-Pheno V2 platform provides an alternative solution for high-throughput phenotyping of low individual plants and is especially suitable for shoot architecture-related plant breeding and management studies.
The lack of efficient crop phenotypic measurement methods has become a bottleneck in the field of breeding and precision cultivation. However, high-throughput and accurate phenotypic measurement could accelerate the breeding and improve the existing cultivation management technology. In view of this, this paper introduces a high-throughput crop phenotype measurement platform named the LQ-FieldPheno, which was developed by China National Agricultural Information Engineering Technology Research Centre. The proposed platform represents a mobile phenotypic high-throughput automatic acquisition system based on a field track platform, which introduces the Internet of Things (IoT) into agricultural breeding. The proposed platform uses the crop phenotype multisensor central imaging unit as a core and integrates different types of equipment, including an automatic control system, upward field track, intelligent navigation vehicle, and environmental sensors. Furthermore, it combines an RGB camera, a 6-band multispectral camera, a thermal infrared camera, a 3-dimensional laser radar, and a deep camera. Special software is developed to control motions and sensors and to design run lines. Using wireless sensor networks and mobile communication wireless networks of IoT, the proposed system can obtain phenotypic information about plants in their growth period with a high-throughput, automatic, and high time sequence. Moreover, the LQ-FieldPheno has the characteristics of multiple data acquisition, vital timeliness, remarkable expansibility, high-cost performance, and flexible customization. The LQ-FieldPheno has been operated in the 2020 maize growing season, and the collected point cloud data are used to estimate the maize plant height. Compared with the traditional crop phenotypic measurement technology, the LQ-FieldPheno has the advantage of continuously and synchronously obtaining multisource phenotypic data at different growth stages and extracting different plant parameters. The proposed platform could contribute to the research of crop phenotype, remote sensing, agronomy, and related disciplines.
The field phenotyping platforms that can obtain high-throughput and time-series phenotypes of plant populations at the 3-dimensional level are crucial for plant breeding and management. However, it is difficult to align the point cloud data and extract accurate phenotypic traits of plant populations. In this study, high-throughput, time-series raw data of field maize populations were collected using a field rail-based phenotyping platform with light detection and ranging (LiDAR) and an RGB (red, green, and blue) camera. The orthorectified images and LiDAR point clouds were aligned via the direct linear transformation algorithm. On this basis, time-series point clouds were further registered by the time-series image guidance. The cloth simulation filter algorithm was then used to remove the ground points. Individual plants and plant organs were segmented from maize population by fast displacement and region growth algorithms. The plant heights of 13 maize cultivars obtained using the multi-source fusion data were highly correlated with the manual measurements ( R 2 = 0.98), and the accuracy was higher than only using one source point cloud data ( R 2 = 0.93). It demonstrates that multi-source data fusion can effectively improve the accuracy of time series phenotype extraction, and rail-based field phenotyping platforms can be a practical tool for plant growth dynamic observation of phenotypes in individual plant and organ scales.
Background The morphological structure phenotype of maize tassel plays an important role in plant growth, reproduction, and yield formation. Plant organ segmentation can be achieved with high-precision and automated acquisition of maize tassel phenotype indicators because of the advances in the point cloud deep learning method. However, this method requires a large number of data sets and is not robust to automatic segmentation of highly adherent organ components; thus, it should be combined with point cloud processing technology. Results An innovative method of incomplete annotation of point cloud data was proposed for easy development of the dataset of maize tassels,and an automatic maize tassel phenotype analysis system: MaizeTasselSeg was developed. The top point cloud of tassel branch was automatically segmented based on PointNet + + network. Complete branch segmentation was realized based on the shortest path algorithm. The Intersection over Union(IoU), precision, and recall of the segmentation results were 96.29, 96.36, and 93.01, respectively. Six phenotypic indexes related to morphological structure (branch count, branch length, branch angle, branch curvature, tassel volume, and dispersion) were automatically extracted from the segmentation point cloud. The squared correlation coefficients (R2) for branch length, branch angle, and branch count were 0.9897, 0.9317, and 0.9587, respectively. The root mean squared error (RMSE) for branch length, branch angle, and branch count were 0.529 cm, 4.516°, and 0.875, respectively. Conclusion The proposed method provides an efficient scheme for high-throughput organ segmentation of maize tassels and can be used for the automatic extraction of phenotypic indicators of maize tassels. In addition, the incomplete annotation approach provides a new idea for morphology-based plant segmentation.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.