Lung adenocarcinoma (LAD) is a common malignancy; however, its underlying molecular mechanism is unclear. Circular RNAs (circRNAs) serve as significant cancer regulators. The overexpression of circRAPGEF5 in LAD tissues and cells indicated that it may be involved in promoting LAD progression. Analysis of 61 LAD tissues revealed that circRAPGEF5 was related to lymph node metastasis. Functionally, circRAPGEF5 promoted the proliferation, migration, invasion, and epithelial-mesenchymal transition of LAD cells in vitro and promoted LAD cells growth in vivo. Mechanistically, dual-luciferase reporter assays confirmed direct interaction of circRAPGEF5, miR-1236-3p, and ZEB1. miR-1236-3p was upregulated and ZEB1 expression reduced after circRAPGEF5 knockdown, and the proliferation, migration, and invasion of LAD cells was inhibited. circRAPGEF5 was significantly overexpressed in LAD cell exosomes, and co-culture experiments showed that exosomal circRAPGEF5 enhanced the metastatic ability of LAD cells. Further experiments found that serum exosomal circRAPGEF5 was overexpressed in LAD; moreover, the area under the receiver operator characteristic curve of exosomal circRAPGEF5 was superior to that of serum carcinoembryonic antigen (CEA). Jointly detected serum exosomal circRAPGEF5 and serum CEA had better diagnostic performance than when detected individually. Thus, exosomal circRAPGEF5 could promote the proliferation and metastasis of LAD via the miR-1236-3p/ZEB1 axis and serum exosomal circRAPGEF5 may serve as a promising biomarker for LAD.

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Sepiella maindroni was once one of the four traditional marine products in our country. In order to evaluate the ecological capacity of Sepiella maindroni in the sea area of Zhongjieshan Islands, this study used Ecopath with Ecosim 6.5 (EwE) software to construct an Ecopath model composed of 17 functional groups in the sea area of Zhongjieshan Islands. The nutrient structure and energy flow characteristics of the sea area of Zhongjieshan Islands are analyzed from the ecosystem level, and the reasonable ecological capacity of the sea area of Zhongjieshan Islands is estimated by the model.The results show that the trophic level of each functional group in the marine ecosystem of the Zhongjieshan Islands is between 1-4.648, total primary production/total respiration is 4.539, the system Connectance Index is 0.478, the System Omnivory Index is 0.389, The trophic level of Sepiella maindroni is 3.562, and the Ecotrophic Efficiency is 0.306, indicating that the Ecotrophic Efficiency of each trophic level in the system is low and the energy is not fully utilized. There is still room for breeding and exile for the Sepiella maindroni. The model estimates that the ecological capacity of Sepiella maindroni is about 2t·km2.