Background Breast cancer (BC) is one of the most prevalent cancers worldwide but its etiology remains unclear. Obesity is recognized as a risk factor for BC, and many obesity-related genes may be involved in its occurrence and development. Research assessing the complex genetic mechanisms of BC should not only consider the effect of a single gene on the disease, but also focus on the interaction between genes. This study sought to construct a gene interaction network to identify potential pathogenic BC genes. Methods The study included 953 BC patients and 963 control individuals. Chi-square analysis was used to assess the correlation between demographic characteristics and BC. The joint density-based non-parametric differential interaction network analysis and classification (JDINAC) was used to build a BC gene interaction network using single nucleotide polymorphisms (SNP). The odds ratio (OR) and 95% confidence interval (95% CI) of hub gene SNPs were evaluated using a logistic regression model. To assess reliability, the hub genes were quantified by edgeR program using BC RNA-seq data from The Cancer Genome Atlas (TCGA) and identical edges were verified by logistic regression using UK Biobank datasets. Go and KEGG enrichment analysis were used to explore the biological functions of interactive genes. Results Body mass index (BMI) and menopause are important risk factors for BC. After adjusting for potential confounding factors, the BC gene interaction network was identified using JDINAC. LEP, LEPR, XRCC6, and RETN were identified as hub genes and both hub genes and edges were verified. LEPR genetic polymorphisms (rs1137101 and rs4655555) were also significantly associated with BC. Enrichment analysis showed that the identified genes were mainly involved in energy regulation and fat-related signaling pathways. Conclusion We explored the interaction network of genes derived from SNP data in BC progression. Gene interaction networks provide new insight into the underlying mechanisms of BC.

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A reasonable width of the coal pillar is very important to the surrounding rock control of the gob-side roadway. An unreasonable width of the coal pillar will make the roadway to be located within the range of strong mining influence, leading to severe deformation of the roadway. Severe subsidence at the coal pillar side of the roof and serious coal pillar deformation are problems caused by strong dynamic pressure due to mining in the gob-side coal roadway. This paper studies the surrounding rock instability mechanism and rational coal pillar width of the gob-side coal roadway under the influence of intense dynamic pressure. The results show that: (1) Under the condition of large mining height, the roadway overburden is a hinged structure, and an unreasonable coal pillar width makes the gob-side coal roadway to be located below the main roof fracture line. The rotary movement of the key block of the main roof is the main reason for the roadway deformation. (2) According to the evolution law of stress field, displacement field and plastic zone of surrounding rock of roadway under different coal pillar widths during roadway driving and mining were studied, and it is concluded that a 6-m-width coal pillar is the most reasonable. (3) Based on the stress distribution and plasticizing range of surrounding rock in a narrow pillar roadway, the combined support scheme of "anchor cable + grouting + single prop" is proposed and applied to engineering practice. The practice results show that the roadway deformation is well controlled, and the safe mining of the working face is realized.

Considering the serious asymmetric deformation and failure of the floor of 1# main roadway in Wanglou Coal Mine, the mechanism of roadway asymmetric floor heave was studied through on-site investigation, theoretical analysis, numerical simulation, and on-site test. The following conclusions were drawn: (1) the floor heave of 1# main roadway is mainly caused by high original rock stress, surrounding rock stress, water physical effect, support strength, etc. (2) A mechanical model of asymmetric floor heave is built and analyzed. Roadway floor stability is relevant to the stress concentration coefficient of the roadway sides, the burial depth of the roadway, and the cohesion and internal angle of friction of the floor rock. The relationship between the upward resultant force of the floor and the stress concentration coefficient of the roadway sides is established. Affected by mining, the upward force of roadway floor reaches 17.0 MPa, and serious floor heave is easy to occur when the floor is opened. (3) The floor heave curve of the position of 1# main roadway corresponding to working face is obviously asymmetric, the maximum value of floor heave being 948 mm. The floor heave curve of other positions of 1# main roadway is basically symmetric, the maximum floor heave value being merely 497 mm. A new “differentiated” combined support is proposed and field tested. It has been 13 months since the completion of main roadway repair in this section, no obvious deformation occurred, and the long-term stability of soft rock roadway support in deep mines is realized.

In order to mater the reasonable layout basis of the terminal mining line position in the repeated mining of close-distance thick coal seams, taking Yan mine as the engineering background, we conducted theoretical analyses, numerical simulations, and field measurements to study the action mechanism of the stress arch. The results show that (1) with repeated mining, the shape of the left and right half arches of the stress arch changes in the order of time and space until it is stable; (2) the shape of the stress arch is highly related to the distribution of abutment pressure in the working face. If the shape is unchanged, the distribution of abutment pressure is unchanged; (3) in the final mining stage of repeated mining, when the shape of the right half arch is stable, the difference stagger distance of the terminal mining line has little effect on the distribution of abutment pressure of the working face where the front arch foot is located; (4) when the internal stagger distance between 3216 working face and terminal mining line of 4216 working face is greater than 22 m or the external stagger distance is greater than 30 m, 3216 working face is located in a relatively safe position. This study clarifies the key factors for the layout of terminal mining lines in close-distance thick coal seams, which can provide a scientific basis for similar projects.