Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid tra⁄cking and metabolism. To better understand its tissue-speci¢c regulation during zebra¢sh hepatogenesis, we isolated 5P P-£anking sequences of the zebra¢sh L-FABP gene and used a green £uorescent protein (GFP) transgenic strategy to generate liver-speci¢c transgenic zebra¢sh. The 2.8-kb 5P P-£anking sequence of zebra¢sh L-FABP gene was su⁄cient to direct GFP expression in liver primordia, ¢rst observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42^51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development. ß

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