Choline is essential in diets fed to most young vertebrates, but previous studies did not confirm the essentiality of choline in diets fed to tilapia. Two experiments were conducted to evaluate the essentiality of dietary choline in such diets. The basal diet used in both experiments contained 32 g crude protein/100 g diet (10.1 g crude protein from casein and gelatin, and 21.9 g from a crystalline L-amino acid mixture). The total sulfur amino acid (TSAA) concentration of the basal diet was 0.28 g/100 g diet, Met:Cys 89:11. In Experiment 1, a 4x2 design was used in which crystalline L-methionine was added to the basal diet resulting in four levels of TSAA (0.28, 0.50, 0.75 or 1.0 g/100 g diet, Met:Cys 89:11, 94:6, 96:4, or 97:3, respectively). At each level of TSAA, diets also contained either 0 or 1 g choline/kg diet supplied as choline chloride. Weight gain, feed efficiency (FE) and serum methionine concentrations were significantly affected by dietary TSAA concentration, but not by dietary choline concentration or the interaction between TSAA and choline. Weight gain, feed efficiency and serum methionine concentrations indicated that the TSAA requirement was 0.5 g/100 g diet. In the second experiment, diets were formulated to contain either 0.28 or 0.5 g TSAA/100 g diet, Met:Cys 89:11 or 94:6, respectively, and graded levels of choline ranging from 1 to 4 g/kg diet in gradations of 1 g/kg. Dietary methionine significantly affected weight gain and FE, whereas dietary choline significantly affected weight gain, FE and survival, and the interaction of methionine and choline significantly affected weight gain. Fish fed diets containing 0.5 g TSAA/100 g diet and 3 g choline chloride/kg diet exhibited the highest weight gain, feed efficiency and survival. On the basis of these data, it seems clear that juvenile tilapia require choline in certain dietary formulations.

Visceral leishmaniasis is a severe chronic disease of people and animals. The disease is caused by several subspecies of a protozoal organism, Leishmania donovani. If not treated, visceral leishmaniasis is often fatal. The most commonly used chemotherapeutic agents to treat the disease are pentavalent antimonials, which can be toxic, must be administered by parenteral routes, and are sometimes ineffective. In this study, meglumine antimoniate, a pentavalent antimony, was compared with WR 6026, an 8-aminoquinoline derivative, as to antileishmanial efficacy. The results indicate that either of these 2 drugs are effective in the suppression of amastigotes in the liver and spleen of the opossum. Despite the marked parasite suppression in the liver and spleen of the infected opossums, the experimental disease was fatal in all of the infected opossums, regardless of the therapy.

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