Thern~ophiles and coliforln bacteria were present in the intestinal tract of certain arctic animals and were sometilnes found in soil and water. Thermophiles survived for a longer period than coliform bacteria when they were inoculated into soil and water simultaneously, and therefore, under natural conditions, some of the thermophiles found in these materials may originate from animal and human feces even though a sample may give a negative test for the presence of colifor~n bacteria.
Reports from several laboratories (Happold and Hoyle, 1936; Epps and Gale, 1942) have demonstrated that tryptophanase activity is reduced when cells are harvested from a medium containing glucose and that this effect is not
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