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To date, only one adverse reaction to alphazurine 2G has been reported.4 A burned patient who received 6 ml of a 10% solution of alphazurine 2G intravenously suffered immediate sneezing, respiratory distress, shock, convulsions, and temporary cardiac arrest. He died five days later. On the other hand, recent reviews5,6 of experiences with lymphography have not mentioned adverse reactions from the dye, which was used to identify lymphatic vessels. The present paper describes the occurrence of nonfatal, anaphylactic reactions to alphazurine 2G in two patients during lymphography.Report of Cases CASE 1.\p=m-\A 56-year-old cheese maker was well until early 1965, when he noted swelling of the right inguinal area. In January 1966 an excisional biopsy of the mass revealed Hodgkin's disease. The patient was referred to the Madison Veterans Administration Hospital on Feb 2 for recommendations for treatment. The medical his¬ tory was unremarkable, and there was no history of allergic disease.The patient appeared healthy. Except for one palpable cervical lymph node, the physical examination showed no abnormality. The hematocrit value was 45%. White blood cell count was 4,500/cu mm, with normal differential cell count. Roentgenograms of chest and of gastrointestinal tract were normal. The pathologist reviewed the biopsy specimen and concurred with the diagnosis of Hodgkin's disease.Two days after admission the patient was referred to the University of Wisconsin Hospitals for lymphography of the intra-abdominal lymph nodes. Two milliters of a 2% solution of lidocaine and 0.25 ml of a 11% solution of alphazurine 2G (Patent Blue V) were injected into both feet. The patient noted immediate itching of the legs, generalized urticaria, nasal congestion, choking, and syn¬ cope. There was no wheezing or hypotension. The patient received dyphenhydramine and hydrocortisone intrave¬ nously and the symptoms gradually diminished. The fol¬ lowing day he had fully recovered. No treatment was recommended, and the patient was discharged eight days after admission. Case 2.-A 40-year-old automobile mechanic entered the Madison (Wis) VA Hospital on Jan 17, 1966, because of abdominal pain. In October 1965, he had noted mild lower abdominal discomfort, relieved by micturition. In Decem¬ ber he had felt vague left upper quadrant abdominal pain. He had lost 6.4 kg (14 lb) in three months. The history was otherwise unremarkable.Physical examination showed a healthy-appearing man with phimosis. Two of several examiners felt a deep mass in the left umbilical region. Findings of laboratory tests were normal. Roentgenograms of gastrointestinal tract and in intravenous pyelogram showed no significant abnormality. Cystoscopic examination showed a meatal stricture and minimal chronic prostatis.The patient was referred to the University of Wisconsin Hospitals for lymphographic examination of the intraabdominal lymph nodes. After intradermal injection of 2 ml of a 2% solution of lidocaine and 2 ml of a 11% solution of alphazurine 2G in both feet, the patient ...

Antibodies (AbMVM) were produced in rabbits to microvillous membranes isolated from hamster small bowel. Incubation of frozen sections of hamster small bowel with fluorescein-labeled AbMVM showed specific reaction with brush borders, but not with other intestinal cellular components. Electron microscopy with ferritin-conjugated AbMVM localized the antigens more precisely to the surface mucopolysaccharide coat of the brush borders. AbMVM also reacted with the brush border of colon and of proximal renal tubules of hamsters but not with those of hamster stomach or gall bladder. It also reacted with the brush borders of some rat and human tissues, but not with those of rabbits. In addition, fluorescent-labeled AbMVM combined specifically with cell walls of some yeasts, but not of several bacteria. AbMVM also contained a weak precipitin to a component of hamster serum, which migrated like prealbumin in immunoelectrophoresis.