No abstract

A phospholipid which rapidly accumulates radioactivity from [1‐14C] acetate administered to slices of developing soybeans or to suspension cultures of soybean cells was isolated. Its structure was identified by comparison of its properties and degradation products with those of authentic lipid standards using infrared absorption spectrometry, thin layer chromatography, acetolysis, mild alkaline hydrolysis, and determination of molar ratios of phosphorus, glycerol, and acyl moieties. The structure of the phospholipid was found to bebis‐phosphatidic acid, a phospholipid previously unreported in higher plants.

Abstractbis‐Phosphatidic acid from developing soybean was completely hydrolyzed with phospholipase A2 (lyophilized venom from Ophiophagus hannah) to yield lyso‐bis‐phosphatidic acid and free fatty acids. It is suggested that soybean bis‐phosphatidic acid is derived from sn‐3‐glycerophospho‐sn‐3′‐glycerol.