Aim: The purpose of this study was to examine the effects of flavonoids in MeOH: DMSO (1:1, v/v) extract lime peel against MRSA. Material and Methods: This study used MeOH: DMSO (1:1, v/v) extract from lime peel in concentrations of 6.25, 12.5, 25, 50, and 100ppm as treatment, penicillin-G as positive control, and DMSO 10% as negative control. This study consisted of five stages, ie. lime plant determination, lime fruit collection, lime peel preparation, extraction, antibacterial potential prediction of flavonoid compounds in extract, in vitro potential antibacterial tests. In in vitro test, each treatment was repeated 3 times and the data obtained were statistically tested using one-way ANOVA. Results: Prediction of the potential of 12 flavonoid compound in lime peel carried out through PASS SERVER showed as many as eight compounds based on prediction in computation that have antibacterial activity with a Probability activity value ≥0.6 and the other four compounds have antibacterial activity with a Probability activity ≤0.6. In vitro test data using one-way ANOVA showed significant differences between variations in concentration (p<0.05). Duncan test showed that the concentration of 100ppm resulted in a significant difference from the concentrations of 50ppm, 25ppm, 12.5 ppm and 6.25ppm, while the concentrations of 6.25ppm, 12.5ppm and 25ppm showed no significant difference. MeOH: DMSO (1:1, v/v) extract of lime peel in concentrations of 6.25, 12.5, 25, and 50ppm resulted in inhibition zone diameter area belonging to resistant category, while at a concentration of 100ppm the inhibition zone diameter area belonged to weak category Conclusion: The MeOH: DMSO (1:1, v/v) extract of lime peel has bacteriostatic potential.