Antibiotics are drugs that work to kill and or inhibit bacterial growth. Antibiotics are a class of prescriptions drugs that are widely used in the management of pharmacological therapy. Antibiotic knowledge must be used rationally and requires knowledge for the patient. Low levels of knowledge about the use of antibiotics can lead to inappropriate use of antibiotics, so there is a risk of causing side effects of antibiotics. Providing education is one way that can be used to increase knowledge about rational use of antibiotics. The aim of this research was to determine the effect of education on the use of antibiotics on outpatients at Kanjuruhan Hospital Malang Regency. This type of research was an Quasi-experimental by using one group pre-test post-test and prospective with sampling using accidental sampling technique data collection of 62 respondents. The result of the research showed that in pre test 44% respondents were having lack antibiotic-use knowledge, 35% respondents were having sufficient antibiotic-use knowledge, and 21% respondents were having good antibiotic-use knowledge. In post-test, 3% respondents belong to low-category, 15% respondents belong to medium-category, and 82% respondents belong to high-category. The T-test showed differences in scores between before and after being given education with a significance value of 0.00 (p value <0.05). The conclusion of this research is that there is an effect of providing education on the level of knowledge of outpatients in Kanjuruhan Hospital Malang Regency.

Objective: This study was aimed to evaluate the role of genistein or 4',5,7-trihydroxyisoflavone as a phytoestrogen in the treatment of estrogen deficiency-induced neuroinflammatory. The specific objectives of this study were to determine the anti-neuroinflammatory effect of genistein through measurement of MHC II and Arg1 expressions on microglia HMC3 cell line, as well as to prove that the effect occurs in an ER-dependent manner, through the measurement of free-ERβ expression. Methods: The cells were cultured in 24-well microplates, induced with 10 ng IFN-γ, and incubated for 24 h to activate the cell to M1 phenotype which has pro-inflammatory characteristics. Genistein with a concentration of 50 μM was added to the cells. The expression of MHC II, Arg1, and free-ERβ as markers was tested through an immunocytochemistry method and measured using the CLSM instrument. In silico approach was also conducted to determine the interaction between genistein and ERβ, compared to 17β-estradiol. Genistein structure was prepared with Avogadro 1.0.1, and molecular docking was done using PyRx 0.8 software. Biovia Discovery Studio Visualizer 2016 was used to visualize the structure of genistein against 3OLS protein. The physicochemical characteristics of genistein were analyzed using the SwissADME web tool. Results: Genistein can decrease MHC II expression and increase Arg1 expression in microglia HMC3 cells compared to negative controls (p<0.005), with expression value of 472.577±26.701 AU and 114.299±6.578 AU. But, genistein cannot decrease the free-ERβ expression in cells (p<0.005). The results of in silico analysis showed that genistein is an ERβ agonist. Conclusion: Genistein shows anti-neuroinflammatory effects by decreasing the MHC II expression and increasing Arg1 expression in the microglia HMC3 cell line. However, this effect does not occur through the binding of genistein to ERβ, but it is likely to occur through the binding of genistein with other types of ER.

Abstract—Urinary Tract Infection (UTI) was the presence of the large number of microorganisms in the urine that can cause urinary tract infections. UTIs were infection whose incidence is still high in Indonesia. The main therapy of UTIs was using antibiotics. The immense of antibiotic use can cause irrationality. This irrationality would give some negative effects such as allergic reaction, physiological changes, and antibiotic resistance. The purpose of this study was to know the quality of antibiotic use in UTI patients in Kanjuruhan Region General Hospital. The type of research was observational by the cross-sectional design. The data were taken retrospectively during September-November 2019 and used the medical records of 27 UTI inpatients. The results of this study determined 20% including category 0 (approriate use of antibiotic), 2,86% category I (antibiotic use not correct timing); 28,57% category II A (antibiotic use not correct dose); 34,29% category II B (antibiotic use not correct interval); 11,42% category III B (antibiotic use is too short) and 2,86% category IV A (there is more effective alternative). Keywords: antibiotic, gyssens, urinary tract Abstrak—Infeksi Saluran Kemih (ISK) merupakan suatu keadaan dimana terdapat mikroorganisme dalam urin yang jumlahnya sangat banyak dan mampu mengakibatkan infeksi pada saluran kemih. ISK tergolong penyakit infeksi yang angka kejadiannya masih tinggi di Indonesia. Terapi utama ISK adalah terapi dengan menggunakan antibiotik. Meluasnya penggunaan antibiotik dapat menyebabkan tidak rasionalnya penggunaan antibiotik. Penatalaksanaan terapi antibiotik yang tidak rasional dapat menimbulkan risiko antara lain adalah reaksi alergi, toksisitas, perubahan fisiologi, dan resistensi antibiotik. Tujuan penelitian ini adalah untuk mengetahui kualitas penggunaan antibiotik pada pasien ISK di RSUD Kanjuruhan Kabupaten Malang. Jenis penelitian adalah observasional dengan menggunakan desain penelitian cross-sectional. Pengambilan data dilakukan secara retrospektif selama bulan September-November 2019 dan menggunakan 27 data rekam medik pasien ISK Rawat Inap. Hasil evaluasi kualitatif menunjukkan, sebesar 20% termasuk kategori 0 (penggunaan antibiotik tepat/bijak); 2,86% kategori I (penggunaan antibiotik tidak tepat waktu); 28,57% kategori II A (penggunaan antibiotik tidak tepat dosis); 34,29% kategori II B (penggunaan antibiotik tidak tepat interval pemberian); 11,42% kategori III B (penggunaan antibiotik terlalu singkat); dan 2,86% kategori IV A (ada antibiotik lain yang lebih efektif). Kata kunci : antibiotik, gyssens, infeksi saluran kemih

Sambiloto (Andrographis paniculata Nees) contains andrographolid compound which has potentialas an anticancer. The difference of growth location has affected the distinction of metabolite content. Thisstudy aims to determine the profile of anticancer activities of A. paniculata extract from several locations inEast Java. The extracts from several locations were tested for activity on T47D cancer cell using MTT assaymethod. Thus, the result of this study describes that A. paniculata extract from several different locations doesnot provide significantly different anticancer activities with the amount of (p>0.05). The extracts which havemoderate anticancer activity were A. paniculata extracts from Selopuro Subdistrict Blitar Regency, SingosariSubdistrict Malang Regency, Lowokwaru Subdistrict Malang City, Klojen Subdistrict Malang City,Purwodadi Subdistrict Pasuruan Regency, Purwosari Subdistrict Pasuruan Regency, and PandaanSubdistrict Pasuruan Regency

Abstract—Pneumonia was an inflammation that occurs in the lungs accompanied by exudation and consolidation of microorganisms. In Indonesia, the prevalence of pneumonia continues to increase. Treatment of pneumonia uses antibiotic therapy. The use of antibiotics needs to be controlled by evaluating the quality of antibiotic use to prevent negative effects that can occur on patients, such as antibiotic resistance. The purpose of this study was to determine the quality of antibiotic use in pneumonia patients. This study did with observational method with cross-sectional design. The study was conducted in August-September 2019. Qualitative evaluations did with Gyssen method. The results of 35 medical record samples that fulfill the criteria showed that 21.43% of cases category 0; 16.67% of cases category I; 0% of cases category II C; 33.34% of cases in category II B; 4.76% of cases category II A; 0% of cases category III B; 0% of cases category III A; 0% of cases category IV D; 19.05% of cases category IV C; 4.76% of cases category IV B; 61.90% of cases category IV A; 0% of cases category V; 0% of cases category VI. Thus, rational cases (category I) were 21,43% and irrational cases (categories I-IV) were 78,57%.