Some whales caught incidentally or illegally in Korean waters have been introduced into the seafood market. Thus, the Korea Coast Guard (KCG) monitors and reports incidentally caught whales. However, species identification of some carcasses based on morphological features is difficult and often inaccurate, particular for processed whale products in the marketplace. For these reasons, we developed a DNA microarray that distinguishes 9 species of cetacean animals for quick and simple species identification. We verified genetic variations in whales and designed species-specific oligonucleotide probes by sequence analysis of mitochondrial cytochrome c oxidase subunit I. Fluorescent dye-labeled DNA targets were generated with a fluorescent dye-labeled primer and hybridized onto a DNA microarray consisting of short probes that were 29-37 nucleotides in length. A DNA microarray system using species-specific probes was successful at rapidly identifying 9 species of whales on the basis of hybridization patterns. Figure 1. Variable nucleotide sites in 446-bp sequences of the cytochrome c oxidase subunit I gene in whales. The dots indicate the nucleotide identity of the Balaenoptera acutorostrata sequence.
A microarray has been developed to distinguish aphid species. DNA microarray technology provides a rapid, easy, cost-effective and accurate method for identifying aphid species for pest control management.
Two species of filefish, Spephanolepis cirrhifer and Thamnaconus modestus, are commonly dried, seasoned with sugar and salt, roasted, and sold as a snack called Jwipo in Korea. These species of fish are imported from China, Thailand, and Vietnam because of drastic catch reduction. However, imported materials have been identified as Aluterus monoceros, Paramonnacathus choirocephalus, and T. septentrionalis in a market survey. For these reasons, we developed a DNA microarray that distinguishes five species of filefish for quick and simple species identification. Species-specific oligonucleotide probes were designed by sequence analysis of mitochondrial cytochrome c oxidase subunit I. In this study, a DNA microarray system using species-specific probes successfully and rapidly identified five different filefish species, and shown potential for determining geographical origin. 230 BioChip J. (2011) 5(3): 229-235 Figure 1. Variable nucleotide sites in 614-bp sequences of the cytochrome c oxidase subunit I gene in filefishes. The dots indicate the nucleotide identity of the Spethanolepis cirrhifer sequence.
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