Steady-state circular RNAs (circRNAs) have been mapped to thousands of genomic loci in mammals. We studied circRNA processing using metabolic tagging of nascent RNAs with 4-thiouridine (4sU). Strikingly, the efficiency of circRNA processing from pre-mRNA is extremely low endogenously. Additional studies revealed that back-splicing outcomes correlate with fast RNA Polymerase II elongation rate and are tightly controlled by cis-elements in vivo. Additionally, prolonged 4sU labeling in cells shows that circRNAs are largely processed post-transcriptionally and that circRNAs are stable. Circular RNAs that are abundant at a steady-state level tend to accumulate. This is particularly true in cells, such as neurons, that have slow division rates. This study uncovers features of circRNA biogenesis by investigating the link between nascent circRNA processing and transcription.

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We study the thermodynamics of modified black holes proposed in the context of gravity's rainbow. A notion of intrinsic temperature and entropy for these black holes is introduced. In particular for a specific class of modified Schwarzschild solutions, their temperature and entropy are obtained and compared with those previously obtained from modified dispersion relations in deformed special relativity. It turns out that the results of these two different strategies coincide, and this may be viewed as a support for the proposal of deformed equivalence principle.