PprI is a general switch protein that regulates the expression of certain proteins involved in pathways of cellular resistance in the extremophilic bacterium Deinococcus radiodurans. In this study, we transformed pprI into Lactococcus lactis strain MG1363 using the lactococcal shuttle vector pMG36e and investigated its effects on the tolerance and lactic acid production of L. lactis while under stress. PprI was stably expressed in L. lactis as confirmed by western blot assays. L. lactis expressing PprI exhibited significantly improved resistance to oxidative stress and high osmotic pressure. This enhanced cellular tolerance to stressors might be due to the regulation of resistance-related genes (e.g., recA, recO, sodA, and nah) by pprI. Moreover, transformed L. lactis demonstrated increased lactic acid production, attributed to enhanced lactate dehydrogenase activity. These results suggest that pprI can improve the tolerance of L. lactis to environmental stresses, and this transformed bacterial strain is a promising candidate for industrial applications of lactic acid production.

Cervical cancer, one of the high-incidence female malignant tumors, has predominated in recent years. Persistent infection with high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer. Studies have shown that infection with certain sexually transmitted disease (STD) pathogens increases the risk of persistent infection with HR-HPV and is a high-risk factor for cervical cancer. In the present study, cervical specimens were collected for Thinprep cytology test detection, while DNA of cervical cells was extracted for HPV genotyping and detection of 10 STD pathogens, including Neisseria gonorrhoeae , Chlamydia trachomatis (CT), Ureaplasma urealyticum , Ureaplasma urealyticum parvum (Uup)1, Uup3, Uup6, Uup14, Mycoplasma hominis (Mh), Mycoplasma genitalium (Mg) and herpes simplex virus II. Significant differences were observed between CT, Mh and Mg infections and HR-HPV infection (P<0.05). In addition, CT, Uup3, Uup6 and Mh infections were associated with HR-HPV infection (odds ratio >1; P<0.05). In the comparison of Uup3, Uup6 and Mg infections between the cervical intraepithelial neoplasia (CIN) group and the control group, statistically significant differences were observed (P<0.05). In conclusion, the incidences of CT, Mh and Mg infections were similar with HR-HPV infection. CT, Uup6, Mh and Mg infections were risk factors for HR-HPV infection. Finally, Uup3, Uup6 and Mg were risk factors of CIN.

The purpose of the study is to analyze the current situation of ideological and political education and psychological education of college students, and explore the challenges faced by these two kinds of education in the information age. First, different research methods, such as literature research, questionnaire survey, and interdisciplinary research, are used to investigate and study the current situation of ideological and political education and psychological education of college students. Second, the survey data are analyzed to reveal the challenges they have encountered. The results show that there are many problems in ideological and political education, which need to be improved and strengthened. The Internet has a strong attraction for college students, but the purpose of getting online of many college students is not clear. Although it plays an important role in improving teaching quality by helping college students establish correct attitudes toward their study, values, life, and society, ideological and political education lack strong pertinence and effectiveness due to the professionalism of the teachers. And the negative impact of network information on ideological and political education of college students is also obvious. About 11.4% of the respondents believe that network information affects their physical and mental health. About 8.4% of the respondents believe that harmful information on the network is easy to induce students to behave wrongly. About 41% believe that computer games distract them from learning, which indicates that the negative impact of network information on college students cannot be underestimated, and it is imperative to strengthen the ideological and political education of college students. The research provides a useful reference for the ideological and political and psychological education in the information environment and helps to solve the problem in the ideological and political education and psychological education of college students.