Mycotoxin is an important contaminant in food and the environment. The conventional methods for detoxification of mycotoxins are plagued by high chemical consumption, secondary pollution, and specific equipment required. In this study, we propose a chemoenzymatic cascade reaction for mycotoxin removal in an effective and green manner using an enzyme-metal hybrid catalyst synthesized by compartmental coimmobilized glucose oxidase (GOx) and Fe 3 O 4 nanoparticles (NPs) on a flower-shaped covalent organic framework (COF). The GOx-Fe 3 O 4 @COF hybrid catalyst exhibits excellent activity in mycotoxin removal due to the enrichment of mycotoxins in COF and the cooperative catalysis between GOx and Fe 3 O 4 NPs. The degradation efficiency of aflatoxin B 1 (AFB 1 ) in the chemoenzymatic cascade reaction catalyzed by GOx-Fe 3 O 4 @ COF is 3.5 times higher than that in the Fenton reaction catalyzed by Fe 3 O 4 @COF. The GOx-Fe 3 O 4 @COF hybrid catalyst is highly active in a wide pH range of 3.0−7.0, overcoming the limitation of the Fenton reaction that can only perform below pH 3.0. This study provides a powerful tool for the efficient removal of mycotoxins.

Aflatoxin B 1 (AFB 1 ) contamination is an important issue for the safety of edible oils. Enzymatic degradation is a promising approach for removing mycotoxins in a specific, efficient, and green manner. However, enzymatic degradation of mycotoxins in edible oil is challenging as a result of the low activity and stability of the enzyme. Herein, a novel strategy was proposed to degrade AFB 1 in peanut oil using an amphipathic laccase−inorganic hybrid nanoflower (Lac NF−P) as a biocatalyst. Owing to the improved microenvironment of the enzymatic reaction and the enhanced stability of the enzyme structure, the proposed amphipathic Lac NF−P showed 134-and 3.2-fold increases in the degradation efficiency of AFB 1 in comparison to laccase and Lac NF, respectively. AFB 1 was removed to less than 0.96 μg/kg within 3 h when using Lac NF−P as a catalyst in the peanut oil, with the AFB 1 concentration ranging from 50 to 150 μg/kg. Moreover, the quality of the peanut oil had no obvious change, and no leakage of catalyst was observed after the treatment of Lac NF−P. In other words, our study may open an avenue for the development of a novel biocatalyst for the detoxification of mycotoxins in edible oils.