Mountain torrent is one of major natural disasters for humans. With the development of the Internet of things, the instrumented terminals, through which the host PC could obtain the real-time water and rainfall information and could alert to a coming disaster, have been widely applied to mountain torrent warning system. In order to ensure the effectiveness of network alerting, the program in the terminals need to be updated for some specific situations, but large quantities of terminals are usually built in complex terrain and fragmented geographically, so that it is difficult and expensive to update or repair the programs. In this paper, a method of remote update, realized on the BAM-4300 hydrologic remote terminal to update the complex programs via GPRS modules, was highly reliable. Experiments show that this method is of safety and reliability, and cumbersome processes of onsite update and maintenance could be avoided.

In this study, the design and development of an integrated control system for microfluidic PCR analysis instrument was presented. PID temperature control algorithm is used. The micro pumps, micro valves, and micro-mixers are command controlled by PC serial port to work together for samples and reagents driving. Sequential control strategy is introduced to control micro-pumps, micro valves, mixers, high-voltage module, and PCR reaction temperature. Labview as a software development platform is utilized to achieve human-computer exchange. All these designs are aimed to achieve the PCR reaction continuously on line from DNA extraction, purification, amplification to detection. An effective design idea for the coupling of complex microfluidic chip and instrument control was providing.

For the precision rotor position control of stream selector, a control system based on direct current motor (DC motor) has been constructed. The DC motor, with a high-precision incremental encoder used as the driving force, was assembled with the stream selector rotor through a shaft coupling. Following the motor rotation, the encoder generated two-channel quadrature pulses and one channel index pulses. An ultralow-power consumption microcontroller (msp430f2232) received theses pulses and calculated them. The position of the slot was determined by the number of pulses counted from the index pulse. Operator can set and monitored the slot positions of five stream selectors simultaneously through the program which was written with LabVIEW on the host computer. This module featured high reliability and low power consumption compared with the one driven by step motor. Beyond that, it was much smaller and lighter.

Chromatographic stationary phases with specific capturing phosphoproteins is widely used in biological sample pretreatment. However, when captured protein is released, it is required to change the pH of the mobile phase or to use an eluent. Usually, the mobile phase or eluent are salt solutions with high concentration and extreme pH or toxic organic reagents. In this situation, these reagents will destroy the activity and structure of phosphorylated proteins. In addition, the mobile phase after switching the column takes longer time to restore the balance, reducing the experimental efficiency. In order to solve the these problems, we introduce temperature-reponsive materials into the chromatographic stationary phase to achieve the capture and release of phosphorylated proteins by changing the temperature only, in which we use water as the mobile phase. This approach overcomes the drawbacks of traditional methods, and makes the separation process safe and simple. Based on the surface initiated Reversible Addition Fragmentation Chain Transfer Polymerization (SI-RAFT) method, silica@pNIPAAm-nanoTiO2, a kind of Metal Oxide Affinity Chromatography, was synthesized by the rapid introduction of functional groups. The synthesis of silica@pNIPAAm-nanoTiO2was confirmed by infrared and X-ray photoelectron spectroscopy. The grafting rate and the lowest critical temperature were measured by TG and DSC. The results showed that the material had qualified temperature-sensitive properties. The grafting conformation and mobile phase pH of the material were optimized before testing the properties and found that when the material grafting ratio was 10% -15%, the graft density was 30%, and the mobile phase pH was 6, it had the best separate effect. Finally, the material successfully achieved the capture and release of adenosine triphosphate and casein phosphopeptides.