Abstract: Determination of multi-elements were studied in the food cooked with medicinal herbs Ophiopogon japonicus（OJ） and Lotus seeds by ICP-AES after HNO3-H2O2 digestion with microwave. The average recoveries are in 91.7%~ 102.4% of As, Cr, Se, Cd, Cu, Mn, Zn, Al, Ba, Fe, Ni, respectively. The relative standard deviation(RSD)is less than 2.8% except Al （9.4%）and detection limit is in the range of 0. 10µg/L ~8.5µg/L except Al （79.6µg/L）. The difference was very obvious（p0.001,except As） that the elements’ content on the comparison OJ with Lotus seeds. There was rich of the trace elements Cu, Zn. The harmful element Al was on the high value（214.7µg/g）in the OJ. It is of important significance to assess the value of the food cooked with medicinal herbs by determination of multiply elements in Lotus seeds, and OJ with ICP-AES. Keyword: multi-elements, the trace elements, the harmful elements, Ophiopogon japonicus, Lotus seeds, ICP-AES