The expression of thioredoxin mRNA is significantly lower in the liver of rats with NAFLD and might reach the lowest point after developing simple fatty liver. Meanwhile the downregulation of thioredoxin mRNA may cause the inflammatory injury initially in NAFLD.

The objective of the present research was to establish a rapid analytical method for paeoniflorin and moisture in Xiaoyao Pills (condensed) by near-infrared spectroscopy. The near-infrared spectral data of 97 samples was collected by Nicolet 6700 NIR spectrograph,and the reference value of index component content were obtained by HPLC and oven-drying method. Then the multivariate calibration model of paeoniflorin and moisture were established by patrical least square (PLS) and predicting the content of unknow samples. The results showed that the correlation coefficients (R2) of the quantitative calibration model for paeoniflorin and moisture were 0.99774,0.95352, the root-mean-square error of calibration (RMSEC) were 0.00489,0.132,the root-mean-square error of prediction (RMSEP) were 0.00827,0.177. The results indicated that NIRS can provide a simple and accurate way for the fast determination of index component in large numbers of Xiaoyao Pills (concentrated).

To establish a rapid analytical method for Loganin in Qiju Dihuang Pills (condensed) by Near-infrared Diffuse Reflectance Technique. Collecting NIR spectra by NIR Diffuse Reflectance Spectroscopy, the partial least square calibration model was built. The correlation coefficients (R2) and the root-mean-square error of cross-validation (RMSECV) were 0.99764 and 0.09340, respectively. In the external validation,coefficients of determination (r2) between NIRS and HPLC values was 0.97348,the root-mean-square error of prediction (RMSEP) was 0.08491. The results showed that the method was rapid, accurate, and could be applied to the fast determination of Loganin in Qiju Dihuang Pills (condensed).

In this paper, the content of forsythoside A and ethanol-extract were rapidly determinated by near-infrared reflectance spectroscopy (NIRS). 85 samples of Forsythiae Fructus harvested in Luoyang from July to September in 2012 were divided into a calibration set (75 samples) and a validation set (10 samples). In combination with the partical least square (PLS), the quantitative calibration models of forsythoside A and ethanol-extract were established. The correlation coefficient of cross-validation (R2) was 0.98247 and 0.97214 for forsythoside A and ethanol-extract, the root-mean-square error of calibration (RMSEC) was 0.184 and 0.570, the root-mean-square error of cross-validation (RMSECV) was 0.81736 and 0.36656. The validation set were used to evaluate the performance of the models, the root-mean-square error of prediction (RMSEP) was 0.221 and 0.518. The results indicated that it was feasible to determine the content of forsythoside A and ethanol-extract in Forsythiae Fructus by near-infrared spectroscopy.

Traditional Chinese medicine(TCM) preparation has a long history in China，and now it is an important direction at the development of hospital preparations, because lacking of quality control technology, quality of the different production batch is not quite stable in the process of production. Therefore, to establish perfect, accurate, rapid quality evaluation method is the key to realize modernization of the traditional Chinese medicine preparation[1,2]. The paper establishes the quantitative analysis model for Tanshinone IIA in Jingutongxiao Pills by near-infrared spectral technology. Through the discussion of spectral wavelength, spectral pretreatment and principal component count, it was found that the second derivative combined with partial least squares (PLS) establishes a best quantitative calibration model. The correlation coefficient of calibration (R2)was 0.975 37, the Root Mean Square Error of Cross Validation (RMSECV) is 0.001 17 and the external prediction deviation (RMSEP) is 0.00174. It indicated that the near-infrared spectral technology could be used for rapid, accurate, nondestructive determination of Tanshinone IIA in Jingutongxiao Pills[3,4].