The carbon isotope fractionation value (Δ) has been widely used to infer the intrinsic water use efficiency (iWUE) of C3 plants. Currently, the most commonly used iWUE method (expressed as iWUEtra) in tree-rings assumes that the mesophyll conductance in plants is infinite. However, many observation-based studies have pointed out that such an assumption leads to overestimating the impact of carbon dioxide (CO2) on iWUE in plants. In this study, a constant gs/gm ratio (0.79) was introduced for calculating iWUE (expressedas iWUEmes). We applied this iWUEmes model to our newly developed intra-annual (10 samples per ring) Δ 13 C chronologies of Cryptomeria fortunei tree for 1965-2017 at Gu Mountain Area and our annual Δ 13 C chronology of Pinus massoniana tree for 1865-2014 at Niumulin Natural Reserve in southeast China. Using dendrochronology techniques, our analysis revealed that the current iWUEtra model overestimates the iWUE values by approximately 2 times and that the iWUE value of trees inferred from iWUEmes modelling decreased significantly in summer-autumn time, which likely indicate that alternative factors play a role in limiting the degree of iWUE improvement under the drought-stressed forest in southeast China.

The snow cover extent (SCE) on the southeastern Tibet Plateau (SETP) has an important impact on the dynamics of the East Asian winter monsoon and the runoff changes of the first and third largest rivers in Asia, namely, the Yangtze River and the Yarlung Zangbo River. Unfortunately, the shortness of instrumental SCE data of a few decades limits our ability to understand its long-term variability before the industrial era. Here, we developed Abies faxoniana tree-ring total ring width (TRW), early-wood width (EWW), and late-wood width (LWW) chronologies for the past four centuries at Little Qamdo Village (XQDV), Markam County, on the SETP. The most significant positive correlation (r = 0.62, p < 0.01) was found between the EWW chronology and SCE from March to May (SCE3–5). The SCE would affect the onset of the growing season through soil moisture, restricting the early-wood growth of trees. Thus, we presented a reconstruction of SCE3–5via EWW chronology since AD 1660 for SETP. We observed two abrupt changes from low to high around the years 1685 and 1998 for our reconstructed SCE3–5. In addition, we found that the positive anomalies of the reconstructed SCE3–5 after 1988 cohered with the distinct increase of the East Asian winter monsoon.

The carbon isotope fractionation value (Δ) has been widely used to infer the intrinsic water use efficiency (iWUE) of C3 plants. Currently, the most commonly used iWUE method (expressed as iWUE tra ) in tree rings assumes that the mesophyll conductance in plants is infinite. However, many observation-based studies have pointed out that such an assumption leads to overestimating the impact of carbon dioxide (CO 2 ) on intrinsic water use efficiency in plants. In this study, a constant g s /g m ratio (0.79) was introduced for calculating iWUE (expressedas iWUE mes ). We applied this iWUE mes model to our newly developed intra-annual (10 samples per ring) Δ 13 C chronology of Cryptomeria fortunei tree for 1965–2017 at Gu Mountain Area and our annual Δ 13 C chronology of Pinus massoniana tree for 1865–2014 at Niumulin Natural Reserve in southeast China. Using dendrochronology techniques, our analysis revealed that the current iWUE tra model overestimates the iWUE values by approximately 2 times and that the iWUE value of trees inferred from iWUE mes modelling decreased significantly in summer-autumn time, which may indicate that alternative factors play a role in limiting the degree of iWUE improvement under the drought-stressed forest in southeast China.