Background information. Substantial evidence indicates the existence of NCSCs (neural crest-derived stem cells) in embryonic mandibular processes; however, they have not been fully investigated or isolated. The aim of the present study was to isolate stem cells from mandibular process during embryonic development by MACS (magneticactivated cell sorting). The findings show that the cells are multipotent and self-renewing.Results. LNGFR (low-affinity nerve-growth-factor receptor) + cells were isolated from rat embryonic mandibular processes by MACS. The cells were grown in clonal culture by limiting dilution to assess their developmental potential. Clone analysis indicated that, first, LNGFR + cells are multipotent, being able to generate at least neurons and Schwann cells, similar to peripheral neural crest stem cells. Secondly, multipotent LNGFR + cells generate multipotent progenies, indicating that they are capable of self-renewal and therefore are stem cells. Thirdly, manipulation of the medium supplementation alters the fate of the isolated LNGFR + cells. Conclusions.These results indicate that LNGFR antibodies label NCSCs with high specificity and purity, and suggest that positive selection using these antibodies may become the method of choice for obtaining multipotent cells from rat embryonic mandibular processes for tissue engineering or regenerative therapeutic use.

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Ovarian cancer has a high mortality, and RNA methylation plays a role in several cancers. Our study aimed to explore the effects of differential expression of TRMT6, an N1-methyladenosine writer, on ovarian cancer and its upstream regulatory mechanism. Preliminary bioinformatics analysis revealed that TRMT6 was differentially expressed in ovarian cancer and normal ovarian tissues. Patients with ovarian cancer and high TRMT6 expression had a poor prognosis, and the area under the receiver operating characteristic curve was 0.759. This finding suggests that TRMT6 may be used as a biomarker for ovarian cancer. We used immunohistochemistry to verify the differential expression of TRMT6 in ovarian cancer and normal ovarian tissues. The differential expression of TRMT6 in ovarian cancer cell lines A2780 and OVCAR3 was confirmed using qPCR and western blotting. CCK-8, transwell assay results suggested that overexpression of TRMT6 promoted the proliferation and migration of ovarian cancer cells, whereas overexpression of miR-191-5p reduced these effects. Results of dual-luciferase reporter gene assays indicated that TRMT6 was the target gene of miR-191-5p. In conclusion, TRMT6 promoted the proliferation and migration of ovarian cancer cells, and its upstream miR-191-5p targeted and regulated TRMT6 to reduce these effects. TRMT6 gene expression may be used as a prognostic biomarker and its regulation as a therapeutic target in ovarian cancer.

Objective. This study is aimed at exploring the effects of the concept of fast-track surgery (FTS) combined with empathic nursing on perioperative complication prevention and postoperative recovery of gynecological malignant tumor patients. Methods. A total of 180 patients with gynecological malignant tumor treated by surgery in our hospital from September 2018 to October 2021 were randomly divided into three groups: group A, group B, and group C, with 60 patients in each group. Group C was given conventional nursing intervention, group B was given FTS intervention, and group A was given FTS intervention combined with empathic care. Psychological stress state (systolic blood pressure, diastolic blood pressure, and heart rate), postoperative recovery indicators (time of first anal exhaust, time of first defecation, time of getting out of bed, and length of postoperative hospital stay), postoperative complications, and psychological state (Self-Rating Anxiety Scale (SAS) and Self-Rating Depression Scale (SDS)) levels of patients in the three groups were compared. Results. Systolic blood pressure, diastolic blood pressure, and heart rate in group A were lower than those in group B, and those in group B were lower than those in group C (all P < 0.05 ). The time of first anal exhaust, time of first defecation, time of getting out of bed, and postoperative hospital stay in groups A and B were shorter than those in group C (all P < 0.05 ).The incidence of postoperative complications in groups A and B was 8.33% and 8.33%, respectively, lower than 23.33% in group C (both P < 0.05 ). SAS and SDS scores of group A were lower than those of groups B and C after intervention (all P < 0.05 ). Conclusion. The concept of FTS combined with empathic nursing is beneficial to reduce the stress response of patients with gynecological malignant tumor before surgery, reduce the risk of postoperative complications, improve patients’ mood, and speed up the rehabilitation process of patients.

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