The NiCo/NiO–CoOx ultra-thin layered catalyst exhibits high-performance towards H2 generation from N2H4·H2O without alkali as a catalyst promoter at 25 °C.

The volume-dependent rotational diffusion coefficient of gold nanorod was used to monitor the formation of protein corona in homogeneous solution in real time. The detection of particle thickness change could reach subnanometer sensitivity.

Liquid−liquid phase separation (LLPS) underlies the formation mechanism of membraneless biomolecular condensates locally to perform important physiological functions such as selective autophagy, but little is known about the relationship between their dynamic structural organization and biophysical properties. Here, a dark-field microscopy based single plasmonic nanoparticle tracking (DFSPT) technique was introduced to simultaneously monitor the diffusion dynamics of multiple gold nanorod (AuNR) probes in a protein LLPS system and to quantitatively characterize the spatiotemporal heterogeneity of the LLPS condensates during their phase transformation. Based on spatially and temporally resolved analysis of the diffusional behavior of the AuNRs, structure and material properties of p62 condensates, such as the viscoelasticity, the compartmentalization, and the recruitment of protein-covered nanoparticles into the large droplet, have been observed. Moreover, the nonsmooth droplet interface, its solidification after further phase transition or maturation, and the size effect of the inner vacuoles have also been revealed. Our method can be potentially applied to in vitro investigation of different reconstituted membrane-free biomolecular condensates and in vivo study of their dynamic evolution.

A high‐speed darkfield microscope has been developed to monitor the rapid rotation of single gold nanorods (AuNRs) and used to study the spatiotemporal heterogeneity of chemical reactions in free solution. A wide range of viscosities from 237 cP to 0.8 cP could be detected conveniently. We studied H2O2 decomposition reactions that were catalyzed by AuNRs coated with Pt nanodots (AuNR@PtNDs) and observed two different rotational states. The two states and their transitions are related to the production and the amalgamation of O2 nanobubbles on the nanorod surface depending on H2O2 concentration. In addition, the local fluidic environment of pure water was found to be non‐uniform in time and space. This technique could be applied to study other chemical and biochemical reactions in solution.