<i>In situ</i> detection of protein corona on single particle by rotational diffusivity

Lin, Xijian; Pan, Qi; He, Yan

doi:10.1039/c9nr06072c

Cited by 20 publications

(24 citation statements)

References 45 publications

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“…In addition, the hydrodynamic radius of PC-ENP complexes in biofluid can be analyzed by 19 F diffusion-ordered NMR (Carril et al, 2017), fluorescence correlation spectroscopy (Shang and Nienhaus, 2017) and high-speed dark-field microscopy (Lin et al, 2019). Molecular motifs can be detected by flow cytometry combined with microfluidics in biological milieu (Lo Giudice et al, 2016).…”

Section: Characterization Of In Vivo Pc Formationmentioning

confidence: 99%

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Bai

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Understanding the basic interactions between engineered nanoparticles (ENPs) and biological systems is essential for evaluating ENPs’ safety and developing better nanomedicine. Profound interactions between ENPs and biomolecules such as proteins are inevitable to occur when ENPs are administered or exposed to biological systems, for example, through intravenous injection, oral, or respiration. As a key component of these interactions, protein corona (PC) is immediately formed surrounding the outlayer of ENPs. PC formation is crucial because it gives ENPs a new biological identity by altering not only the physiochemical properties, but also the biobehaviors of ENPs. In the past two decades, most investigations about PC formation were carried out with in vitro systems which could not represent the true events occurring within in vivo systems. Most recently, studies of in vivo PC formation were reported, and it was found that the protein compositions and structures were very different from those formed in vitro. Herein, we provide an in-time review of the recent investigations of this in vivo PC formation of ENPs. In this review, commonly used characterization methods and compositions of in vivo PC are summarized firstly. Next, we highlight the impacts of the in vivo PC formation on absorption, blood circulation, biodistribution, metabolism, and toxicity of administered ENPs. We also introduce the applications of modulating in vivo PC formation in nanomedicine. We further discuss the challenges and future perspectives.

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Section: Characterization Of In Vivo Pc Formationmentioning

confidence: 99%

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Bai

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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“…From these time-dependent intensity profiles, the azimuth and polar angles of AuNRs in every frame could be calculated. The orientation distribution pattern obtained through this information, together with the rotational diffusion coefficients, D r , obtained from the autocorrelation analysis of the AuNR intensities, 34,35,42 the centroid trajectories, and the translational diffusion coefficients, D 2 , allowed us to reveal unprecedented details of the orientation states and state transitions of AuNRs during their entire cellular internalization process.…”

Section: Details Of the Whole Cellular Internalization Process Of Aunrsmentioning

confidence: 99%

“…Here, we investigated the cellular internalization dynamics and kinetics of single AuNRs by following their translational and rotational motions at cell sidewall by dual-channel polarization microscopy, according to our previously reported technique. [33][34][35] Although the number of AuNRs experiencing cellular internalization, observable at cell sidewall, is relatively smaller than on cell top, the whole process could be captured readily, with an improvement of the angular precision. Particularly, we integrated the azimuth and polar angles into a polar coordinate system and found three general orientation distribution patterns closely associated with cellular internalization dynamics, which provided tips to aid in confirming one-dimensional contact of the nanomaterials with the membrane by tips after landing on the cell surface.…”

Section: Introductionmentioning

confidence: 99%

Unveiling Cellular Internalization Dynamics of Single Gold Nanorods by Tracking Their Orientational and Translational Motions

Xü

et al. 2022

CCS Chem

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Single nanoparticle tracking (SPT) is a unique and powerful tool to investigate the interaction between nanoparticles and cells, which is of considerable importance for nanotechnology applications in biomedical fields and in-depth understanding of biological activities. However, previous work typically focused on translations of single nanoparticles while they undergo both translational and rotational motions. In this study, we obtained both the translational and rotational dynamics of single gold nanorods during their cellular internalization process using dual-channel polarization microscopy. In particular, the azimuth and polar angles were integrated into a polar coordinate system to obtain three general orientation distribution patterns, found to have a close relationship with the nanoparticle cellular internalization process and time-dependent alterations. Moreover, the patterns accompanied by trajectories, translational and rotational coefficients, the azimuth and polar angles, and other parameters provided a wealth of knowledge on the nanoparticle cellular internalization dynamics with unprecedented details. We observed that the gold nanorods could initially assume a tip-first quick rotation state with partially restricted orientations, then change to a strongly confined near-vertical insertion state with slight angular fluctuations, and eventually transform into a random and fast rotation state. Our methodology opens up a new avenue for a detailed understanding of biological processes.

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“…In this study, the thermodynamic parameters of the interactions between gold nanorods and BSA and fibrinogen were determined. Corona formation around gold nanorods in complex media was also researched . They also investigated the dynamic heterogeneity of gold nanorods on plasma membranes in real time by tracking translational and rotational diffusion.…”

Section: Accurate Analysis Applicationmentioning

confidence: 99%

Dark-Field Microscopy: Recent Advances in Accurate Analysis and Emerging Applications

2021

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In situ detection of protein corona on single particle by rotational diffusivity

Abstract: The volume-dependent rotational diffusion coefficient of gold nanorod was used to monitor the formation of protein corona in homogeneous solution in real time. The detection of particle thickness change could reach subnanometer sensitivity.

Cited by 20 publications

References 45 publications

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

In vivo Protein Corona Formation: Characterizations, Effects on Engineered Nanoparticles’ Biobehaviors, and Applications

Unveiling Cellular Internalization Dynamics of Single Gold Nanorods by Tracking Their Orientational and Translational Motions

Dark-Field Microscopy: Recent Advances in Accurate Analysis and Emerging Applications

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