Deschampsia antarctica Desv. is one of two vascular plants from the Maritime Antarctic. It is usually exposed to cold, salt, and desiccating winds. We hypothesize that D. antarctica has genes that encode dehydrin proteins and their expression is regulated by low temperature, salt or osmotic stress. To test this hypothesis a fragment of a dehydrin gene from D. antarctica was identified and used as a probe to study dehydrin expression under low temperature, salt, and osmotic stress, and exogenous ABA (abscisic acid) treatments. An anti-dehydrin antibody was also used to study dehydrin protein accumulation under the same treatments. Southern analysis of genomic DNA treated with different endonucleases showed more than four bands recognized by the probe, suggesting that D. antarctica has several dehydrin genes. Northern analysis showed two putative dehydrin transcripts of 1.0 kb accumulated only under exogenous ABA and 1.6 kb under osmotic and salt treatments, suggesting that D. antarctica would have ABA-dependent andindependent pathways for regulation of dehydrin expression. Western analysis showed seven dehydrin proteins (58, 57, 55, 53, 48, 30 and 27 kDa) under the different stress treatments. Cold-accumulated dehydrin proteins were immunolocalized, showing that they are associated with vascular and epidermal tissue, which are preferential ice nucleation zones.

SummaryWe studied hemostatic and inflammatory cardiovascular risk factors (CVRF), and total plasma homocysteine (tHcy) in 26 vegetarians (23 lacto- or ovolactovegetarians and 3 vegans), matched by age, sex and socioeconomic status with omnivorous controls. Vegetarians had significantly lower proportion of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids in plasma lipids, significantly shortened bleeding time, and increased blood platelet count and in vitro platelet function (aggregation and secretion). Plasma levels of all coagulation or fibrinolytic factors and natural inhibitors synthesized in the liver were lower in vegetarians than in controls. Whereas for some factors this decrease was statistically significant (fibrinogen, factor VIIc, anti-thrombin III, protein S, plasminogen) for the remaining (factors VIIIc, Vc, prothrombin, protein C) a trend in the same direction was found. For hemostatic proteins of predominantly extrahepatic origin (von Willebrand factor, tPA, PAI-1) this tendency was not present. No significant differences in inflammatory proteins (C-reactive protein and α1-protease inhibitor) were detected in both groups. tHcy was significantly increased in vegetarians, and correlated only with cobalamin levels. The increased platelet function and tHcy found in vegetarians may counteract the known cardiovascular health benefits of vegetarian diet (VD).

Ocurrencia de levaduras del género Candida y estomatitis protésica antes y después del tratamiento rehabilitador basado en prótesis removible ଝ Recibido el 9 de enero de 2014; aceptado el 6 de julio de 2014 Disponible en Internet el 1 de abril de 2015 PALABRAS CLAVE Prótesis removible; Estomatitis protésica; CandidaResumen El 63,2% de la población chilena mayor de 65 años utiliza prótesis removible. Cuando estas pierden funcionalidad pueden producirse lesiones en la mucosa oral, siendo la más prevalente la estomatitis protésica, proceso inflamatorio de la mucosa de soporte de diversa extensión y severidad, cuyo principal factor etiológico es la infección por Candida spp. El objetivo de esta investigación fue determinar la cantidad y las especies de levaduras del género Candida y su asociación con estomatitis protésica en portadores de prótesis removible antes y después del tratamiento rehabilitador. Se realizó un estudio descriptivo cuantitativo, n = 34, en ambos géneros, edad promedio de 69 años, portadores de prótesis removible no funcionales, con y sin estomatitis protésica. Para el recuento e identificación de levaduras del género Candida se tomaron muestras de saliva antes y después del tratamiento. Las variables fueron analizadas estadísticamente. Los resultados indicaron el diagnóstico de estomatitis protésica en el 55,9% de los sujetos, de los cuales tipo i = 29,4% y tipo ii = 26,5%. Los recuentos de Candida spp. fueron mayores en aquellos con estomatitis protésica, tanto antes como después del tratamiento. Al

Resumen: Este estudio pretende comprender, a través del análisis del discurso, el compromiso bioético de estudiantes de odontología que participan en el proceso enseñanza-aprendizaje clínico. Mediante una metodología cualitativa se abordó el objeto de estudio en su escenario natural. La recolección de datos se realizó a través de diversas fuentes de información. Los instrumentos utilizados fueron análisis documental, observación no participante y entrevista semiestructurada. Para analizar los datos se identificaron temas significativos, determinando unidades de registro con el fin de categorizar la información. Para comprender los procesos involucrados se presenta información empírica mediante descripción de tendencias. Éstas describen la información empírica utilizando indicadores por categoría. El análisis explicativo reconstruye el sentido de la información obtenida. Se concluye que la comunicación, confianza y responsabilidad son indicadores de escaso desarrollo en el colectivo. El análisis del discurso, desde la perspectiva de los estudiantes, devela diversas visiones de las prácticas docentes relativas al compromiso bioético, planteándose entonces la necesidad de repensarlas para contribuir con una formación profesional de real compromiso social.Palabras clave: bioética, investigación cualitativa, compromiso, odontología Students commitment to bioethics during the process of clinical training in DentistryAbstract: This study pretends to understand, through speech analysis, the commitment to bioethics of dentistry students, who participate in the process of clinical training. Through a qualitative methodology the object of study was approached in its natural setting. Data collection was carried out through diverse information sources. The instruments used were documental analysis, observation without participating and semi structure interviews. In order to analyze data, meaningful topics were identified, determining units to record for categorizing information. To understand the processes involved, empirical information is shown by describing tendencies. These describe empirical information using indicators for each category. The analysis builds the meaning of information recorded. It is concluded that communication, trust and responsibility are indicators of scarce development in general. Speech analysis, under the perspective of students, reveals diverse visions about teaching practices related to bioethics commitment, raising the need to reconsider these practices in order to contribute to a professional training of real social commitment.Key words: bioethics, qualitative research, commitment, dentistry Compromisso bioético dos estudantes durante processo de ensino-aprendizagem clínico em OdontologiaResumo: Este estudo pretende compreender, por meio da análise do discurso, o compromisso bioético de estudantes de odontologia que participam do processo ensino-aprendizagem clínico. Mediante uma metodologia qualitativa se abordou o objeto de estudo em seu cenário natural. A coleta de dados foi realizada por ...