No abstract

Molybdenum disulfide (MoS2) is an emerging material with some unique physical and electronic properties somewhat comparable to those of graphene. It was prepared with the use of a simple hydrothermal process, and has a layered structure similar to that of graphene. Also, it has a peroxidase-like catalytic activity and is able to catalyze the oxidation of the colorless peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB), in the presence of H2O2 to produce a blue product. This reaction used MoS2 as a useful peroxidase, which involved a colorimetric method for trace analysis of H2O2 in water, e.g. lake waters. This method is uncomplicated, inexpensive and highly sensitive for H2O2 in the 0.125-1.75 μM range (LOD: 0.08 μM). It is also quite stable in the presence of many common inorganic and organic potentially interfering compounds, e.g. metal ions as well as amino acids and sugars.

No abstract

Metabolite-protein interactions (MPIs) play key roles in cancer metabolism. However, our current knowledge about MPIs in cancers remains limited due to the complexity of cancer cells. Herein, the authors construct an integrative MPI network and propose a MPI network based hepatocellular carcinoma (HCC) subtyping and mechanism exploration workflow. Based on the expressions of hub proteins on the MPI network, two prognosis-distinctive HCC subtypes are identified. Meanwhile, multiple interdependent features of the poor prognostic subtype are observed, including hypoxia, DNA hypermethylation of metabolic pathways, fatty acid accumulation, immune pathway up-regulation, and exhausted T-cell infiltration. Notably, the immune pathway up-regulation is probably induced by accumulated unsaturated fatty acids which are predicted to interact with multiple immune regulators like SRC and TGFB1. Moreover, based on tumor microenvironment compositions, the poor prognostic subtype is further divided into two sub-populations showing remarkable differences in metabolism. The subtyping shows a strong consistency across multiple HCC cohorts including early-stage HCC. Overall, the authors redefine robust HCC prognosis subtypes and identify potential MPIs linking metabolism to immune regulations, thus promoting understanding and clinical applications about HCC metabolism heterogeneity.