To determine the prevalence of bovine giardiasis in Heilongjiang Province in China and to molecularly characterize Giardia duodenalis, feces were collected from 814 dairy and beef cattle ranging in age from 6 days to 9 years. Clinical symptoms of diarrhea were recorded at the time of sampling. The G. duodenalis infection rate in cattle was 5.2 % (42/814) as determined by Lugol's iodine staining. G. duodenalis assemblages and subtypes were genetically diagnosed by sequence analysis of the triosephosphate isomerase (TPI) gene. Three assemblages were identified, representing A (n = 1), B (n = 18), and E (n = 24), with a mixed infection case of assemblages A and E. High heterogeneity was also observed within assemblages B and E at the TPI locus. Among the assemblages, eight subtypes of assemblage B and three subtypes of assemblage E were found to be novel subtypes. Findings on assemblages A and B are of public health importance. The zoonotic potential of bovine giardiasis needs to be further assessed by extensive genetic data of assemblages A and B from humans at the subtype level. The newly found subtypes of assemblages B and E imply that the evaluation of geographically distributed subtypes is of importance.
Although the COVID-19 epidemic has lasted for months, it has not yet been successfully controlled, and little is known about neonatal COVID-19. Therefore, literature search was conducted for references in PubMed, Science Direct, ProQuest, Web of Science and China National Knowledge Infrastructure for detailed case reports on neonatal COVID-19 published as of July 15, 2020, to facilitate the clinical treatment, epidemic prevention and control of neonatal COVID-19. Forty nonoverlapping case reports focusing mainly on the demographic characteristics, transmission modes, clinical features, treatments and prognosis of neonatal COVID-19, including 3 in Chinese and 37 in English, were available.
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