Background LncRNA was known to be closely associated with the progression of human tumors. The role of lncRNA LIFR-AS1 in the pathogenesis and progression of gastric tumor is still unclear. The aim of this study was to investigate the function of LIFR-AS1 and the underlying mechanism in the pathogenesis and progression of gastric cancer. Methods QRT-PCR was used to evaluate the expression of LIFR-AS1, miR-29a-3p and COL1A2 in gastric tumor tissues and cells. Western blotting was used to evaluate the protein expression of COL1A2 in gastric tumor cells. CCK-8 assay, transwell assay and flow cytometry were used to evaluate the roles of LIFR-AS1, miR-29a-3p and COL1A2 in cell proliferation, invasion, migration and apoptosis. The relationship among LIFR-AS1, miR-29a-3p and COL1A2 was assessed by bioinformatics analyses and luciferase reporter assay. Results The expression levels of LIFR-AS1 were significantly increased in gastric tumor tissues and cells, while the expression levels of miR-29a-3p were decreased. The expression of miR-29a-3p was negatively correlated with the expression of LIFR-AS1 in gastric cancer tumor tissues. Knocking down of LIFR-AS1 inhibited proliferation, invasion and migration of gastric tumor cells, and induced apoptosis of gastric tumor cells. Bioinformatics analyses and integrated experiments revealed that LIFR-AS1 elevated the expression of COL1A2 through sponging miR-29a-3p, which further resulted in the progression of gastric tumor. Conclusion LIFR-AS1 plays an important role as a competing endogenous RNA in gastric tumor pathogenesis and may be a potential target for the diagnosis and treatment of gastric tumor.

Background Test anxiety has been widely found in medical students. Emotion regulation and psychological resilience have been identified as key factors contributing to anxiety. However, studies on relationships were limited. This study investigated the links between psychological resilience, emotion regulation, and test anxiety in addition to exploring the differences about socio-demographic factors. Methods A sample of 1266 medical students was selected through cross-sectional survey from a medical university in China during 2019. Data were obtained by network technique using designed questionnaire, which assesses the level of test anxiety, emotion regulation and psychological resilience, respectively. Results Medical students experienced test anxiety at different levels, 33.7% of these were seriously. It revealed significant effects of the gender and academic performance on test anxiety. Results of logistic regression indicated that test anxiety was significantly associated with emotion regulation and psychological resilience (p < 0.01). Psychological resilience played a mediating role on the relationship between emotion regulation and test anxiety. Conclusions These findings highlight the importance of psychological resilience and emotion regulation in understanding how psychological resilience relates to test anxiety in medical students. Resilience-training intervention may be developed to support students encountering anxiety during the exam.

a b s t r a c tXiashan Reservoir is the largest reservoir in the Shandong peninsula and the major water supply source for urban, industry and agriculture in Weifang, Shandong Province. In the upstream region of Xiashan Reservoir, its industry development ranged in the top three among 12 districts and counties of Weifang were lower than that in 1995 because of improvements in water utilization and a shift in industry structure. Correspondingly, the total industrial wastewater discharged changed little from 1995 to 2004, but the total COD Cr discharged was greatly reduced.

Background: Medical students experience a considerable amount of anxiety due to exams. Emotion regulation and psychological resilience are established protective factors of individual mental health, however, the investigations for the effects of anxiety were limited. The goal of the present study was to examine the relationship of psychological resilience and emotion regulation with test anxiety and the associated factors of them among medical students. Methods: In this cross-sectional survey, a simple random sampling methods was used to select the participants. Information from a sample of 1266 medical students was collected by self-reporting questionnaires. Logistic regression was applied to test the associations between test anxiety and emotion regulation, resilience. Bootstrap were conducted to explore the mediating role of resilience. Results: Our important results were that the prevalence of problematic test anxiety among medical students to be 71.4%, 33.7% was high test anxiety. Gender and academic performance correlated significantly with test anxiety, emotion regulation, and psychological resilience. There were correlations between test anxiety and various dimensions of emotion regulation and psychological resilience ( P<0.01 ). Emotion regulation and psychological resilience predicted emerging test anxiety. The mediating role of psychological resilience was identified for the effects of emotion regulation on test anxiety. Conclusions: Findings suggest that emotion regulation affected test anxiety through psychological resilience, which may provide insights for clinical psychologists, raise their awareness of the importance of cultivating and improving medical students' psychological resilience, and prompt them to offer psychological support to students with test anxiety as early as possible. The combination of curing and self-healing can solve the root cause of the problem and truly apply psychological research to improving the mental health of the general public. Keywords: Psychological resilience, Test anxiety, Emotion regulation, Medical student

Background: To examine the differences between pregnant women who underwent embryo transfer (ET) and those who conceived naturally, as well as differences in their respective babies, and to determine the causes for these differences, to provide recommendations for women who are planning to undergo ET. Methods: A retrospective cohort study was performed of women who had received ET and those who had natural conception (NC) who received medical services during pregnancy and had their babies delivered at the Shunde Women and Children's Hospital of Guangdong Medical University, China between January 2016 and December 2018. In line with the requirements of the ethics committee, before the formal investigation, we first explained the content of the informed consent of the patient to the patient, and all the subjects included agreed to the content of the informed consent of the patient. Respondents agreed to visit and analyze their medical records under reasonable conditions. Each case in an ET group of 321 women was randomly matched with three cases of NC (963 cases) who delivered on the same day. The demographic information, past history, pregnancy and delivery history, and maternal and neonatal outcomes of the two groups were compared using univariate analysis.