Background
Drought has become the major abiotic stress that causes losses in rice yields and consequently is one of the main environmental factors threatening food security. Long non-coding RNA (lncRNA) is known to play an important role in plant response to drought stress, while the mechanisms of competing endogenous RNA (ceRNA) in drought resistance in upland rice have been rarely reported.
Results
In our study, a total of 191 lncRNAs, 2115 mRNAs and 32 miRNAs (microRNAs) were found by strand-specific sequencing and small RNA sequencing to be differentially expressed in drought-stressed rice. Functional analysis of results indicate that they play important roles in hormone signal transduction, chlorophyll synthesis, protein synthesis and other pathways. Construction of a ceRNA network revealed that MSTRG.28732.3 may interact with miR171 in the chlorophyll biosynthesis pathway and affect the ability of plants to withstand drought stress by regulating Os02g0662700, Os02g0663100 and Os06g0105350. The accuracy of the regulatory network was verified by qRT-PCR.
Conclusion
Our results provide a theoretical basis for future studies on the potential function of lncRNA in plant drought resistance, and they provide new genetic resources for drought-resistant rice breeding.
Shanlan upland rice is a kind of upland rice and is suitable for planting in the mountains and in hilly terrain. It is mainly found in China’s Hainan province. To discover the drought-resistant genes in Shanlan upland rice, two representative varieties—Baishanuo (BSN) and Dongfang Manpoxiang (MPX)—were selected for transcriptome sequencing, after which gene expression analysis was used to confirm their gene expression patterns. The results demonstrated that 2791 and 829 differentially expressed genes (DEGs) were identified for each variety, including 184 and 58 transcriptional factors, respectively. Expression analysis demonstrated that some genes with unknown functions, such as Os10g0505900, were highly expressed under drought stress treatment. The transcriptomic data and digital gene expression profiling data obtained in this study provide a basis for studying the drought-resistant mechanism in Shanlan upland rice.
Electromagnetic tube expansion has been given much attention due to its superior cold forming properties. The traditional electromagnetic coil is of single structure, which results in substantial tube wall thinning and mechanical strength reduction. This shortcoming restricts further development of the electromagnetic tube expansion technology in various industrial applications. This paper proposes an axial-radial electromagnetic force bidirectional loading method to increase the axial fluidity of the material and eliminate the wall thinning issue that currently exists in the traditional method. Furthermore, the performances of three proposed structures for the axial compression electromagnetic tube expansion using three-coil, two-coil and one-coil are analyzed and compared. In this context, the electromagnetic force distribution and the wall thinning of the tube during the expansion process are analyzed based on finite element modelling. Results show that with the proposed modified structure of the traditional single coil, the axial electromagnetic force can be substantially increased which results in reducing the tube wall thinning significantly. The proposed technique in this paper paves the way for further development and more industrial applications of the electromagnetic expansion technology. INDEX TERMS Electromagnetic expansion technology, tube wall thinning, axial electromagnetic force, axial compression.
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