Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 2 (BdOLV2), derived from phytopathogenic fungus Botryosphaeria dothidea strain ZM180192-1, infecting maize in Henan province of China. The complete genome sequence of BdOLV2 consists of a positive-sense singlestranded RNA (+ ssRNA) segment with a length of 2,532 nucleotides (nt). The sequence contains a large open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp) including 605 amino acids (aa) with a molecular mass of 68.59 kDa. This RdRp protein contains eight typical conserved domain motifs associated with ourmia-like virus. BLASTp analysis revealed that the RdRp protein of BdOLV2 had the highest similarity (62.10%, 58.15%, and 55.75% identity, respectively) with Botourmiaviridae sp., Macrophomina phaseolina ourmia-like virus 2 and Macrophomina phaseolina ourmia-like virus 2-A. Phylogenetic analysis based on the RdRp aa sequence indicated that BdOLV2 is a new member of the genus Magoulivirus with in the family Botourmiaviridae.

Here, we describe the complete genome sequence and molecular characterization of a novel ourmia-like virus, tentatively named “Botryosphaeria dothidea ourmia-like virus 3” (BdOLV3), isolated from the phytopathogenic fungus Botryosphaeria dothidea strain ZM190712-1, associated with branch dieback of walnut in Henan Province, China. The complete BdOLV3 genome is 2247 nucleotides (nt) in length, with a content of 31.55% G, 16.11% A, 23.54% U, and 28.79% C. The BdOLV3 genome contains a single large open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 612 amino acids (aa) with a molecular mass of 67.68 kDa. This RdRp protein contains a catalytic core domain associated with ourmia-like virus in the family Botourmiaviridae. A phylogenetic tree revealed that the novel ourmia-like virus clustered with members of the genus Magoulivirus and was separate from members of the other eleven genera of the family Botourmiaviridae.

Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 2 (BdOLV2), derived from phytopathogenic fungus Botryosphaeria dothidea strain ZM180192-1, infecting maize in Henan province of China. The complete genome sequence of BdOLV2 consists of a positive-sense single-stranded RNA (+ ssRNA) segment with a length of 2,532 nucleotides (nt). The sequence contains a large open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp) including 605 amino acids (aa) with a molecular mass of 68.59 kDa. This RdRp protein contains eight typical conserved domain motifs associated with ourmia-like virus. BLASTp analysis revealed that the RdRp protein of BdOLV2 had the highest similarity (62.10%, 58.15%, and 55.75% identity, respectively) with Botourmiaviridae sp., Macrophomina phaseolina ourmia-like virus 2 and Macrophomina phaseolina ourmia-like virus 2-A. Phylogenetic analysis based on the RdRp aa sequence indicated that BdOLV2 is a new member of the genus Magoulivirus with in the family Botourmiaviridae.

Here, a novel mycovirus, Botryosphaeria dothidea narnavirus 5 (BdNV5), was discovered in the plant-pathogenic fungus Botryosphaeria dothidea strain ZM210167-1. The BdNV5 genome sequence is 2,397 nucleotides (nt) in length and contains a putative open reading frame (ORF) encoding the RNA-dependent RNA polymerase (RdRp) with a molecular mass of 72.77 kDa. A BLASTp comparison of the RdRp sequence showed that it was most similar to the RdRp Botryosphaeria dothidea narnavirus 4 (42.35%). The phylogenetic tree based on RdRp sequence showed that BdNV5 clusters together with the members of the family Narnaviridae. BdNV5 is thus a novel member of the family Narnaviridae from the phytopathogenic fungus B. dothidea.