Cryptococcus neoformans is a medically important fungus and can infect all the organs of the body. As vascular endothelial cell is an important target for C. neoformans to penetrate any organs, the differential protein expression of human umbilical vascular endothelial cell (HUVEC) after incubating with C. neoformans may be the key to penetration. The proteins of HUVECs incubated with C. neoformans and normal HUVECs were collected and purified. After two-dimensional electrophoresis, the differential protein expression was identified by matrix-assisted laser desorption ⁄ ionisation mass spectrometry. The mRNA levels of some proteins were measured by real-time PCR. Three proteins were found significantly overexpressed in HUVECs incubated with C. neoformans, and nine other proteins were downregulated. The mRNA levels of S100A10 and peroxiredoxin I fluctuated with the protein levels. These results suggested that the expressions of peroxiredoxin I and S100A10 were regulated during the process of invasion of HUVECs by C. neoformans. We hypothesise that these proteins take part in the modifications of HUVEC cytoskeleton and the tolerance to oxidative stress, which may affect the process of invasion by C. neoformans.

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Nowadays, multi-modal discourse, including language, picture, audio and video play a very important role in the translation of folk songs. Under the comprehensive framework of Delu Zhang, this paper analyses the application of multi-modal discourse in the translation of Tujia folk song Long Chuan Diao from four aspects: cultural level, context level, content level and expressive level. The study can better understand Tujia folk songs and transmit Tujia culture. Multi-modal discourse analysis plays an important role in translating and spreading Tujia folk songs in Western Hubei province.