It has been considered that glucose fluctuation (GF) plays a role in renal injury and is related to diabetic nephropathy (DN) development. But the mechanism is still unclear. Aerobic glycolysis has become a topical issue in DN in recent years. There is an internal connection between GF, aerobic glycolysis, and DN. Curcumin (Cur) is a principal curcuminoid of turmeric and possesses specific protective properties in kidney functions. Cur also participates in the regulation of aerobic glycolysis switch. In this study, we first measured the levels of aerobic glycolysis and evaluated Cur’s inhibitory ability in a cell model of HEK-293 under the condition of oscillating high glucose. The results indicated that GF exacerbated inflammation injury, oxidative stress, and apoptosis in HEK-293 cell, while Cur alleviated this cytotoxicity induced by GF. We found that GF increased aerobic glycolysis in HEK-293 cells and Cur presented a dose-dependent weakening effect to this exacerbation. Next, we built a panel of 17 miRNAs and 8 lncRNAs that were previously reported to mediate the Warburg effect. Our RT-qPCR results indicated that GF reduced the miR-489 content in the HEK-293 cell model and Cur could prevent this downregulation. Then, we planned to explore the character of miR-489 in Cur-triggered attenuation of the Warburg effect under GF condition. Our findings presented that Cur prevented GF-triggered aerobic glycolysis by upregulating miR-489 in HEK-293 cells. Next, we choose the miR-489/LDHA axis for further investigation. We confirmed that Cur prevented GF-triggered aerobic glycolysis via the miR-489/LDHA axis in HEK-293 cells. In conclusion, this study presented that Cur prevented GF-triggered renal injury by restraining aerobic glycolysis via the miR-489/LDHA axis in the HEK-293 cell model.

To summarize the nursing experience of 55 cases of Trimeresurus stejnegeri snakebites. A review of 55 cases of snake bites received by our hospital as a designated snake bite hospital, Give emergency treatment and wound care. Nursing critical points included: Partial wound closure treatment, Observation and management of wounds and injured limbs, the anti-venom medicine should be used as early as possible, mental health care, and health promotion; after a series of nursing care, the patients were all cured and released from the hospital.

Oesophageal bezoars are one of the many causes of nasogastric tube obstruction; however, they are extremely rare and, therefore, not often considered to be the cause of a blockage. A bezoar is a solid mass of indigestible material that accumulates in the digestive tract. After a blockage is identified, the nasogastric tube is usually removed and another one inserted. However, this can be dangerous and can easily cause tearing of the oesophageal mucosa, bleeding, and other serious complications. In this article, the authors present a case of nasogastric tube obstruction caused by oesophageal bezoars. After the nasogastric tube was replaced, the patient experienced two tears of the oesophageal mucosa. This article highlights the importance of the introduction of a procedure for nurses to follow in cases of nasogastric tube obstruction, bearing in mind the possibility of the presence of oesophageal bezoars. If necessary, a gastroscope should be used to ensure safe insertion of the nasogastric tube and prevent oesophageal mucosal tears.

Objective To understand the current situation of emergency nurses’ specialty competence and propose improvement strategies accordingly, aiming to provide a reference for the comprehensive enhancement of nurse competency as well as the selection and training of high-level nursing management talents. Methods Using a self-made questionnaire based on literature review, 266 emergency specialist nurses in 15 tertiary general hospitals in Guangdong Province were surveyed through the questionnaire star. Results The survey showed that the participants appeared to have a high level of self-confidence and recognition for most indicators, as suggested by a high score (195.46±23.64), except for the aspect of scientific research ability. The scores of scientific research ability items ranged from 3 to 15 points (the 50th quantile was 9 points), and the score was 10.41±2.28 with a median score of 10, indicating that clinical nurses lacked confidence in scientific research abilities. The results of linear regression analysis suggested that gender, job title, publications in recent 5 years, and whether directly affiliated to a teaching hospital were the influencing factors that affect the competence of emergency nurses. Conclusions Scientific research ability is the major shortcoming of emergency specialist nurses. The influencing factors that affect nurses’ competencies include gender, professional title, publications in recent 5 years and whether directly affiliated to a teaching hospital. Nursing scientific research is the power source of the continuous development of nursing discipline. Therefore, it is recommended that the leaders of healthcare authorities and hospitals should strengthen the training of nursing scientific research and improve the scientific research ability of nurses.

Objective Penicillin allergy skin test is a common procedure in nursing care. Many patients can only do an allergy skin test on fasting due to the disease condition. However, whether or not the fasting state has any effects on the skin testing results remains unclear. Therefore, this study aimed to determine the effects of fasting on the results of penicillin allergy skin tests. Methods This observational cohort study analyzed the clinical data of 200 patients undergoing a penicillin skin test in the emergency center of The Seventh Affiliated Hospital of Sun Yat-sen University from August 1, 2020, to December 31, 2020, of which 100 cases were fasting patients and 100 were non-fasting patients. The information including gender, age, allergy, skin test results, dizziness, and gastrointestinal symptoms were collected and compared. Result Among the 200 patients, five patients (2.5%) tested positive for the penicillin-allergy skin tests. Three patients (3%) tested positive in the fasting group, while two patients (2%) in the non-fasting group tested positive (Relative Risk: 1.5; 95% Confidence Interval: 0.3055 to 7.397; P=0.65). Conclusion These data suggest that fasting may have limited impacts on the results of penicillin allergy skin tests.