The tassel development status and its branch number in maize flowering stage are the key phenotypic traits to determine the growth process, pollen quantity of different maize varieties, and detasseling arrangement for seed maize production fields. Rapid and accurate detection of tassels is of great significance for maize breeding and seed production. However, due to the complex planting environment in the field, such as unsynchronized growth stage and tassels vary in size and shape caused by varieties, the detection of maize tassel remains challenging problem, and the existing methods also cannot distinguish the early tassels. In this study, based on the time series unmanned aerial vehicle (UAV) RGB images with maize flowering stage, we proposed an algorithm for automatic detection of maize tassels which is suitable for complex scenes by using random forest (RF) and VGG16. First, the RF was used to segment UAV images into tassel regions and non-tassel regions, and then extracted the potential tassel region proposals by morphological method; afterwards, false positives were removed through VGG16 network with the ratio of training set to validation set was 7:3. To demonstrate the performance of the proposed method, 50 plots were selected from UAV images randomly. The precision, recall rate and F1-score were 0.904, 0.979 and 0.94 respectively; 50 plots were divided into early, middle and late tasseling stages according to the proportion of tasseling plants and the morphology of tassels. The result of tassels detection was late tasseling stage > middle tasseling stage > early tasseling stage, and the corresponding F1-score were 0.962, 0.914 and 0.863, respectively. It was found that the model error mainly comes from the recognition of leaves vein and reflective leaves as tassels. Finally, to show the morphological characteristics of tassel directly, we proposed an endpoint detection method based on the tassel skeleton, and further extracted the tassel branch number. The method proposed in this paper can well detect tassels of different development stages, and support large scale tassels detection and branch number extraction.

Variety regional tests based on multiple environments play a critical role in understanding the high yield and adaptability of new crop varieties. However, the current approach mainly depends on experience from breeding experts and is difficulty to promote because of inconsistency between testing and actual situation. We propose a spatial layout method based on the existing systematic regional test network. First, the method of spatial clustering was used to cluster the planting environment. Then, we used spatial stratified sampling to determine the minimum number of test sites in each type of environment. Finally, combined with the factors such as the convenience of transportation and the planting area, we used spatial balance sampling to generate the layout of multi-environment test sites. We present a case study for maize in Jilin Province and show the utility of the method with an accuracy of about 94.5%. The experimental results showed that 66.7% of sites are located in the same county and the unbalanced layout of original sites is improved. Furthermore, we conclude that the set of operational technical ideas for carrying out the layout of multi-environment test sites based on crop varieties in this paper can be applied to future research.

No abstract

No abstract

An appropriate layout of crop multi-environment trial (MET) sites is imperative for evaluating new crop varieties’ performance in terms of agronomic traits and stress tolerance, and this information is used to determine the utilization value and suitable promotion region of new varieties. Actually, traditional maize test sites have been selected according to the experience of breeding experts, which leads to the strong subjective and unscientific conclusions regarding sites, as well as test results that are not representative of the target population of environments (TPE). Therefore, in this study, we proposed a new method for MET sites layout. Meteorological data, maize growth period data, and county-level maize planting area data were collected for the spatiotemporal classification of a given maize planting region to analyze change rules in the environmental category of each minimum research unit within the study period. If the occurrence frequency of its final attribution category reaches a certain threshold (50%), this minimum research unit is classified as a typical environment region; otherwise, it is classified as an atypical environment region. Then, the number of test sites in each environmental category is allocated by spatial stratified sampling. At last, we establish the optimal test sites layout and a reliability measurement (test adequacy) methods. The practicability of this method was proved by taking the Three Northeastern Provinces of China as the study area. The result shows that there should be 112 test sites in the study area, the distribution of the test sites is uniform, and the environmental representation is high. Test adequacy analysis of the test sites reveals that most of the environmental categories have a test adequacy that reaches 1 in each test period. The method proposed in this paper provides support for the scientific layout of crop varieties test sites and helps to improve the representative and reliability of variety test results while optimizing resources.