No abstract

New Delhi Metallo Beta Lactamase 1 (NDM-1) is an enzyme with zinc ions at its active site that cleaves the amide bond of β-lactam ring and provides resistance against major classes of βlactam antibiotics. The molecular detection of NDM-1 producing bacterial isolates from tertiary Hospitals in Kano was investigated. A total of 500 bacterial isolates of Enterobacteriaceae and Pseudomonas aeruginosa from samples of blood, urine, catheter tip were screened for NDM-1 over

Pathogenic fungi have been given lesser consideration in the diagnosis of respiratory tract infections compared to Bacteria. This research was therefore aimed at screening for mycotic agents associated with pulmonary symptoms in patients attending Infectious Diseases Hospital, Kano -Nigeria. Two hundred sputum samples from two hundred patients were collected and investigated for mycotic infections. The samples were cultivated on sabouraud's dextrose agar (SDA) containing Gentamycin and incubated at room temperature and at 37 o C for seven to fourteen days. Dalamau plate technique was employed to differentiate yeast types. Germ-tube test was used to confirm the presence of Candida albicans. Out of one hundred and eleven positive samples, 63.06% were males and 36.94% were females. Forty-One (36.94%) were positive with Aspergillus species, forty (36.04%) with Candida species, fifteen (13.51%) with Geotrichum species, ten (9.01%) with Penicillium species, two (1.80%) with Alternaria species; also Curvularia species (1.80%) and one subject (0.90%) was found to be positive with Sepedonium species. Significant association was found between fungal colonization with age and with antibiotic usage (P<0.05). Since the overall incidence was found to be 55.50%, it implies that fungal culture had helped in the diagnosis of fungal pulmonary disease, however, Aspergillus and Candida species were found more vulnerable in compounding bronchopulmonary disorders.