ABSTRACT:The chelating membranes for adsorption of metal ions were prepared by the bonding of linear and branched polyethylenimines (LPEI and BPEI) on the glycidyl methacrylate (GMA) photografted porous polyethylene (pPE) (pPE-g-PGMA) films. The adsorption and desorption properties of LPEI and BPEI-bonded pPE-g-PGMA (LPEI-(pPE-g-PGMA) and (BPEI-(pPE-g-PGMA)) films to Cu 2þ ions were investigated as a function of the grafted amount, amount of bonded PEI, molecular mass of PEI, pH value, and temperature. The amounts of LPEI and BPEI bonded to the pPE-g-PGMA films increased over the reaction time, and the bonding of LPEI and BPEI offered the water-absorptivity to the pPE-g-PGMA films. The amount of adsorbed Cu 2þ ions at pH 5.0 had the maximum value at the grafted amount of 10 mmol/g for the (LDPEI-(pPE-g-PGMA) and (BPEI-(pPE-g-PGMA) films with a constant amount of bonded PEI. The amount of adsorbed Cu 2þ ions for the LPEI-(pPE-g-PGMA) films was higher than that for the BPEI-(pPE-g-PGMA) films. The amount of Cu 2þ ions desorbed from the LPEI-(pPE-g-PGMA) and BPEI-(pPE-g-PGMA) films increased with an increase in the HCl concentration. The quantities of Cu 2þ ions of about 100% were desorbed in the aqueous HCl solutions of more than 0.1M for the LPEI-(pPE-g-PGMA) films and more than 0.05M for the BPEI-(pPE-g-PGMA) films. The amounts of adsorbed Cu 2þ ions were almost the same in each adsorption process at pH 5.0. This indicates that the LPEI-(pPE-g-PGMA) and BPEI-(pPE-g-PGMA) films can be applied to a repeatedly generative chelating membrane for adsorption and desorption of metal ions.

Previously, many studies have revealed the presence of B chromosomes in wild mouse taxa of the genus Apodemus (Rodentia, Muridae). In one of the Apodemus species, A. argenteus, which is endemic to Japan, it is known that B chromosomes were confirmed only in individuals (2n = 46 + B chromosome) from Hokkaido, Japan. There is no report of the presence of B chromosomes from other localities in the Japanese Islands. In this study, we analyzed the chromosomal constitutions of 43 individuals of A. argenteus from three localities in Honshu, Japan. A total of three individuals from central Honshu showed 2n = 47, and each individual carrieda dot-like B chromosome. In addition, these B chromosome features were analyzed by differential staining methods, and the C- and QM-banding patterns of the B chromosomes were identical to those of the X chromosomal heterochromatic region showing the delayed-fluorescent response. Thus, it is considered that these B chromosomes would be derived from the heterochromatin of the X chromosomes, as reported in previously published papers.

Previously, many studies have revealed the presence of B chromosomes in wild mouse taxa of the genus Apodemus (Rodentia, Muridae). In one of the Apodemus species, A. argenteus, which is endemic to Japan, it is known that B chromosomes were confirmed only in individuals (2n = 46 + B chromosome) from Hokkaido, Japan. There is no report of the presence of B chromosomes from other localities in the Japanese Islands. In this study, we analyzed the chromosomal constitutions of 43 individuals of A. argenteus from three localities in Honshu, Japan. A total of three individuals from central Honshu showed 2n = 47, and each indi vidual carried a dot like B chromosome. In addition, these B chromosome features were analyzed by differ ential staining methods, and the C and QM banding patterns of the B chromosomes were identical to those of the X chromosomal heterochromatic region showing the delayed fluorescent response. Thus, it is consid ered that these B chromosomes would be derived from the heterochromatin of the X chromosomes, as reported in previously published papers.