The yolk content of each poultry varies so that the quality of spermatozoa diluents from various egg yolks will also vary. The purpose of this study was to determine the effect of the use of chicken, duck, and egg yolks-quail in Tris dilution to the quality of Simental spermatozoa. Semen used in this study was semen Simental bulls from at the Tuah Sakato Artificial Insemination center, Payakumbuh, West Sumatera. The research used a completely randomized design (CRD) of three treatments with ten replications. The results of the research showed that the use of different egg yolk diluent gave a significant effect in motility, viability, membrane plasma intact, recovery rate and abnormalities spermatozoa Simental. Conclusion, the duck egg yolk to the tris diluent for Simental bulls spermatozoa preservation is the best diluent with high motility (43.00%), high intact plasma membrane (54.67%), high the viability (45.17%) with low abnormality spermatozoa (13.33%) and lower recovery rate (57.33 %) compared to the tris egg yolk-chicken diluent and tris egg yolk-quail.