The recovery rate of Simental spermatozoa frozen of post thawing by using tris dilution with different egg yolks

Yendraliza, Yendraliza; Hendriyanto, Y.; Mucra, Dewi Ananda; Zumarni,; Rodiallah, Muhammad

doi:10.1088/1755-1315/391/1/012008

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…The percentage of sperm viability in this diluent was 70%, slightly higher than AndroMed® diluent. The percentage of sperm viability of Bali cattle in this study was higher than the sperm viability in Simmental cattle (45.17%) (Yendraliza et al 2019). This difference in viability was due to the difference in diluent composition.…”

Section: Semen Quality During Cryopreservationcontrasting

confidence: 67%

Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

2021

Int J Vet Sci

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The aim of this research was to evaluate the Bali cattle semen quality during cryopreservation with different types of extenders in term of live, total motility, progressive motility, and abnormality of post-thawed bull sperms. The treatments were AndroMed® (T0), Tris-based egg yolk diluent (T1), Tris-based coconut water diluent (T2), Coconut water egg yolk diluent (T3). Bulls’ semen was collected from two adult Bali cattle maintained at the semen production facility at Bali Artificial Insemination Center, Tabanan Bali. The age of the bulls were 6 years old. Sperm live, total motility, progressive motility, and abnormality were analyzed with computer assisted sperm analysis (CASA) post-dilution, before and after thawed. The study was replicated five times, and data were analyzed using analysis of variance (ANOVA). The results showed that AndroMed ® and Tris-based egg yolk had significantly higher sperm live, total motility, progressive motility and abnormalities of spermatozoa for post-dilution, after equilibration and post thawed than Tris egg yolk coconut water and Coconut water egg yolk diluent. It was concluded that AndroMed® and Tris-based egg yolk can be considered as the best suitable extender for Bali cattle sperm cryopreservation. Coconut water had a deleterious effect when supplemented with 20% in tris and egg yolk.

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Section: Semen Quality During Cryopreservationcontrasting

confidence: 67%

Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

2021

Int J Vet Sci

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The comparison of Javanese bull and ongole crossbreed bull semen quality diluted with different diluents

Bintara¹,

Asmarawati²,

Aji³

2023

Proceedings of the 4th International Conference of Animal Science and Technology (Icast 2021)

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The addition of egg yolk to the physiological saline extender improved the motility and viability of kampung rooster spermatozoa at cool temperatures

Diba,

Suzanita Utama,

Tjuk Imam Restiadi

et al. 2023

Ovz

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The purpose of this study was to determine the motility and viability of kampung rooster (Gallus gallus domesticus) spermatozoa in 0.9% Sodium chloride (NaCl) solution as an extender with the addition of egg yolk at different concentrations at cool temperature (5°C). This study was performed using two 1.5 years old healthy local roosters. Semen was collected through massage in the morning. The pooled semen sample was divided into four treatment groups. In the T0 group the semen was diluted in 0.9% NaCl, while in the T1, T2 and T3 groups the semen was diluted in 0.9% NaCl added with 5, 10 and 15% egg yolk. The results showed that the motility and viability of spermatozoa decreased when stored at cool temperatures for ten hours (p <0.05). Semen of roosters stored at 5°C in saline solution without the addition of egg yolks showed the lowest motility and viability of spermatozoa (p <0.05). The addition of egg yolk into the saline extender increased the motility and viability of spermatozoa. Concentration of 15% egg yolk in saline solution resulted in the highest spermatozoa motility and viability when stored for up to 8 hours (p <0.05). However, motility and viability of spermatozoa at 10 hours of storage were not significantly different (p >0.05) with the addition of 10% and 15% egg yolks. Therefore, it could be concluded that the addition of 15% egg yolk into a saline solution as an extender could maintain the motility and viability of kampung rooster spermatozoa when stored at 5°C for 10 hours.

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The recovery rate of Simental spermatozoa frozen of post thawing by using tris dilution with different egg yolks

Cited by 3 publications

References 12 publications

Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

Evaluation of Bali Cattle Semen Quality During Cryopreservation with Coconut Water-Based Extenders

The comparison of Javanese bull and ongole crossbreed bull semen quality diluted with different diluents

The addition of egg yolk to the physiological saline extender improved the motility and viability of kampung rooster spermatozoa at cool temperatures

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