Müllerian-inhibiting substance (MIS) is an essential factor for male sexual differentiation. In the present study, we examined whether the expression of MIS and several of its related transcription factors is altered in the ovaries of rats treated with the synthetic estrogen, E2 benzoate (EB; 10 microg/0.02 ml), from postnatal day 1 (P1) to P5. The EB-treated rats had a significantly reduced number of layered follicles at P6 in comparison with the control rats that were treated with vehicle alone. The expression levels of both MIS mRNA and protein in the granulosa cells of small growing follicles in the ovary at P6 were higher in the EB-treated rats than in the controls. These results indicate that the inhibitory effect of EB on the follicular stratification may correlate with the inappropriately increased expression level of MIS. Furthermore, the expression levels of one of its transcriptional activators, steroidogenic factor 1, and ER-beta in granulosa cells of small growing follicles were higher in EB-treated ovaries than in the control ovaries. These results suggest the role of MIS in the regulation of follicular growth and the possible involvement of steroidogenic factor 1 and/or ER-beta in this molecular cascade may contribute to postnatal ovarian development.
633The inelastic properties of Cu,,Ti,, amorphous specimens are investigate.. .elow 300 K using the flexural resonant vibration in the strain amplitude range 10-5 < E < 10-3. With increasing E, the reversible inelastic component ~i increased at first and tended towards saturation (Ei,t). The general changes in d%/ds can well be described by a Gaussian distribution in E , but in the low E range deviations occur on both sides with a complementary nature. From these results, the number density of deformable sites N ( E ) in the amorphous structure is surmised to have, in general, a Gaussian distribution in E. These features for dsi/de remain the same for various temperatures, and ~j , t increases with decreasing temperature. These facts suggest that atomic rearrangements in deformable sites causing ~i are athermal phenomena. Both plastic deformation and thermal cyclings modify dsi/ds only in the low E range.Die inelastischen Eigenschaften von amorphen Cu,,Ti,,-Legierungen werden mit Hilfe von Resonanz-Biegungsschwingungen unterhalb 300 K im Dehnungsamplitudenbereich gemessen. Mit zunehmendem E nimmt die reversible inelastische Komponente ~i zuniichst zu und tendiert dann zur Slttigung (Ei,t). Die allgemeinen Anderungen in dei/ds konnen gut durch eine GauR-Verteilung in E beschrieben werden, im Bereich kleiner E-Werte treten jedoch auf beiden Seiten Abweichungen von komplementiirer Natur auf. Aus diesen Ergebnissen wird geschlossen, da13 die Dichte der deformierbaren Plltze in der amorphen Struktur eine GauB-Verteilung in E besitzt. Dieses Verhalten von dei/de ist von der Temperatur unabhlngig, E i , t nimmt jedoch mit abnehmender Temperatur zu. Diese Fakten legen den SchluR nahe, daB die atomaren Umverteilungen auf den deformierbaren Plltzen, die die Ursache fur ~i sind, athermischer Natur sind. Sowohl plastische Deformation als auch thermische Wechselbeanspruchung veriindern dsi/ds nur im Bereich kleiner E-Amplituden.
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