The ampulla, isthmus, uterus, and cervix of mixed-age Romney ewes were examined for the presence of spermatozoa 1, 2, 4, 8, 16, 24, 36, and 48 hrs after a single mating. Spermatozoa were recovered from the following sites at the stated intervals after copulation: the cervix 1 hr, the uterus 2 hrs, the isthmus 4 hrs, and the lower ampulla 16 hrs. Peak numbers in all regions occurred 16 hrs after mating. Cervical numbers were noticeably constant up to 16 hrs and then began to fall.Spermatozoa disappeared rapidly from the reproductive tract between 36 and 48 hrs after copulation, probably because of recommencement of normal dioestral flows of luminal fluids.Oxytocin (lI.U.) administered 3t hrs after copulation enhanced the number of spermatozoa reaching the lower ampulla. Synchronisation of oestrus with progesterone appeared to inhibit both the rate of transport of spermatozoa in ewes and the number entering the uterus and isthmus. These findings based on only a small number of animals are discussed in the light of present knowledge on sperm transport in the ewe.