We developed a video-assisted gliding test to evaluate the gliding force and the flexion angle with unrestricted joint motion. Tendon adhesion was induced in a chicken model of flexor digitorum profundus (FDP) injury at the annular pulley region of the long toe. The chicken feet were harvested immediately after injury, and 2 weeks and 6 weeks after injury. During the gliding test, the injured FDP was pulled for 15 mm then returned to its initial position. The test was recorded using a video camera and registered to the gliding test mechanical data. The maximum flexion angle and gliding resistance were calculated. The maximum flexion angle was significantly decreased from 78 (SD 10) in controls to 42 (SD 22) in tendons with injury, while gliding resistance was significantly increased in week 2 (0.06, SD 0.05) and week 6 (0.07, SD 0.01) after injury.