Preeclampsia (PE) is a major cause of the pregnancy morbidity and mortality over the world. Disorganized placentation caused by trophoblast cell abnormity is one of main risk factors to induce PE. MiR-133a-3p has been shown to contain regulatory effects on oxidative stress in the cardiomyocytes. But the effects of miR-133a-3p on oxidative stress-induced apoptosis in the trophoblast cells remain unknown. In this study, trophoblast HTR-8/SVneo cells were transfected with miR-133a-3p mimics and inhibitor. H2O2 (250 μM) treatment of cells was adopted to induce oxidative stress. A series of typical molecular and cellular experiments was subsequently performed in order to investigate this issue. It was found that miR-133a-3p overexpression attenuated the oxidative stress induced by H2O2 through reduced ROS and MDA levels and enhanced antioxidase activities in the trophoblast cells. Overexpressed miR-133a-3p was shown to relieve the oxidative stress-induced apoptosis of HTR-8/SVneo cells. At molecular levels, a direct binding effect of miR-133a-3p on BACH1 was verified. Moreover, miR-133a-3p overexpression also enhanced BACH1 downstream Nrf2/HO-1 signaling to activate antioxidant genes. It is collectively demonstrated that miR-133a-3p can relieve the oxidative stress-induced apoptosis in the trophoblast cells through the BACH1/Nrf2/HO-1 signaling pathway via targeting BACH1 directly. This regulatory mechanism of miR-133a-3p in the trophoblast cells under oxidative stress may give a new perspective for oxidative stress-induced trophoblast cell abnormality and be useful to study more pathological mechanisms of PE.

Purpose: In premise of uninfluenced to dose distribution of tumor target and organ at risk(OAR) in cervical cancer,area of segment fields was changed to increase efficacy and optimize treatment method by designing different plan of intensity modulated radiotherapy(IMRT). Methods: 12 cases of cervical cancer were confirmed in pathology and treated with step and shoot IMRT. Dose of PTV was 50Gy/25fractions. Every patient was designed 9 treatment plans of IMRT by Pinnacle 8.0m planning system,each plan was used with 9 beams of uniform distribution and fixing incidence direction(200°,240°,280°,320°,0°,40°,80°,120°and 160°respectively),and designed for delivery on Elekta Synergy linear accelerator. All plans were optimized with the direct machine parameter optimization(DMPO) algorithm using the same set of optimization objectives. Number of maximum segment field was defined at 80 and minimum MU in each segment was 5MU,and minimal segment area was 2*1cm2,2*2cm2,3*3cm2,4*4cm2,5*5cm2,6*6cm2,7*7cm2,8*8cm2and 9*9cm2,respectively.Coverage,homogeneity and conformity of PTV,sparing of OAR, MU and number of segment were compared. Results: In this group, mean volume of PTV was 916.8±228.7 cm3. Compared with the area of minimal segment field increased from 2*1cm2 to 9*9 cm2,the number of mean MU was decreased from 1405±170 to 490±47 and the number of segment field was reduced from 76±4 to 39±7 respectively(p<0.05). When the limit of minimal segment area was increased from 2*1cm2 to 7*7 cm2,dose distribution of PTV,OAR,CI,HI and V2 3 were not different (p>0.05),but when the minimal segment area was 8*8 cm2 and 9*9 cm2,they were changed compared with 7*7 cm2 and below(p<0.05). Conclusion: The minimal segment field of IMRT plan designed by Pinnacle 8.0m planning system in cervical carcinoma should be enlarge reasonably and minimal segment area of 7*7 cm2 was recommend.

Purpose: To understand the correlation between radiation induced pneumonitis (RIP) and esophagitis (RIE) with dosimetric parameters for patients treated using 3‐D conformal radiation therapy (3DCRT) for non‐small cell lung cancers (NSCLC). Methods: Dosimetric parameters of dose‐volume histograms from 3DCRT plans for 104 NSCLC patients treated between 2000 to 2004 from our hospital were retrospectively analyzed. The prescription doses ranged from 60–78Gy with a median dose of 66Gy. The RIP and RIE were assessed for each patient during the treatment and follow‐up within 3 months after treatment completion. The correlation between dosimetric parameters with RIP and RIE were evaluated by univariate and multivariate analysis using Logistic Regression Model of SPSS 11.0 software. The predictive ability of parameters was assessed with receiver operating characteristic (ROC)curves. The area under the curve (AUC) was used to filter cut‐off values for predictive RIP and RIE. Results: The rate of RIP>=2 grade was 38.3% for patients. Univariate analysis showed that the lung mean dose and volumes receiving doses from 5 to 40Gy (V5–V40)were important factors for predicting RIP. Multivariable analysis indicated that lung V35 was likely to be an independent factor. The optimal cut‐off value for lungV35 was 20.75%, corresponding to sensitivity of 66.10% and specificity of 81.00% in ROC curve. The rate of RIE was 46.2%. Multivariate analysis showed that both GTV and PTV of NSCLC, and esophagus V60 were the independent parameters for predicting RIE. The optimal cut‐off value for esophagus V60 was 12.50%, corresponding to sensitivity of 81.30% and specificity of 69.60% in ROC curve. It was found that there was significantly difference between AUC of ROC curve for esophagus V60 and those for GTVˈs and PTVˈs of NSCLC (P<0.05). Conclusions: lung V35 and esophagus V60 were likely to be the independent factors predicting of RIP and RIE, respectively. Our funding support received from National Natural Science Foundation of China (30870743).

Purpose:In premise of uninfluenced to dose distribution of tumor target and organ at risk(OAR) in Nasopharyngeal carcinoma (NPC),the number of segment was changed to increase efficacy and optimize treatment method by designing different plan of intensity modulated radiotherapy(IMRT).Methods:10 cases of NPC were confirmed in pathology and treated with step and shoot IMRT. The target volumes were defined: GVT‐T (the primary tumor); GVT‐N (the metastasis lymph nodes in neck); CTV‐T and CTV‐N (the clinical targets of GVT‐T and GTV‐N); and PTV (incidental lymph nodal region). Dose of GTV/CTV/PTV was 70/64/54Gy for 30 fractions.Every patient was designed 7 treatment plans of IMRT by Pinnacle 8.0m planning system,each plan was used with 9 beams of uniform distribution and fixing incidence direction(200°,240°,280°,320°,0°,40°,80°,120°and 160°respectively),and designed for delivery on Elekta Synergy linear accelerator.All plans were optimized with the direct machine parameter optimization(DMPO) algorithm.Minimum MU in each segment was defined at 4MU,and minimal segment area was 4cm2.Number of maximum segment field was defined at 120,110,100,90,80,70,60, respectively.Coverage and conformity of GTV/CTV/PTV,sparing of OARs(spinal cord, brain, parotids, lens and optic nerves) were compared.Results:In this group,mean volume of GTV(GTV‐T+GTV‐N),CTV(CTV‐T+CTV‐N)and PTV were 91.39±71.13cm3,177.27±92.16cm3 and 1028.05±167.03cm3,.respectively. Compared with the number of segment decreased from 120 to 60, there were no difference in MU(p>0.05),and the dose distribution of targets(GTV/CTV/PTV),OARs and CI were not different too(p>0.05).But when the segment number was <60, CI of PTV was changed compared with others(p<0.05).Conclusion:The minimal segment number of IMRT plan designed by Pinnacle 8.0m planning system in NPC should be decreased reasonably and the limit of minimal segment number was recommended by 70.