. 2006. In vitro ruminal digestion of anthocyanidin-containing alfalfa transformed with the maize Lc regulatory gene. Can. J. Plant Sci. 86: 1119-1130. In vitro ruminal digestion was conducted on novel "purple-green" alfalfa forage that accumulated anthocyanidin and proanthocyanidins through the expression of a maize bHLH anthocyanidin regulatory gene (Lc). The Lc-transgenic genotypes were compared with parental (non-transformed, NT) alfalfa in their in vitro ruminal fermentation, dry matter (DM) and N disappearances, and DM degradability. The transgenic genotypes expressed the Lc gene at different levels when grown under high light conditions, resulting in anthocyanidin contents as high as 136 µg g -1 DM. Lc genotypes had lower true DM disappearance than NT alfalfa at 0, 4 and 12 h of incubation, but not at 24 or 48 h. Compared with NT, Lc-transgenic genotypes had a lower content of rapidly soluble DM, but a similar content and rate of degradation of the slowly degradable DM fraction, and a similar lag time for digestion. True disappearance of N was lower for Lc-transgenic than NT alfalfa at the initiation of the incubation. The solubility of both DM and N were negatively correlated with the concentration of anthocyanidins measured in the forage. The results indicate that Lc-transformation reduced the initial rate, but not the extent of DM and N digestion of alfalfa in the rumen. These properties could improve the utilization of protein and possibly reduce the risk of bloat in ruminants consuming fresh alfalfa. However, further increasing the amount of anthocyanidins (or proanthocyanidins) produced in the forage may be required to make this a viable strategy for improved protein utilization and bloat prevention. Les auteurs ont examiné la digestion par le rumen in vitro du nouveau fourrage de luzerne « vert violacé » dans lequel l'anthocyanidine et les proanthocyanidines s'accumulent, consécutivement à l'expression du gène régulateur (Lc) de l'anthocyanidine bHLH du maïs. Ils ont comparé les génotypes transgéniques Lc aux lignées parentales (non modifiées, NM) de luzerne pour la fermentation dans le rumen in vitro, la disparition de la matière sèche (MS) et de l'azote (N) ainsi que la dégradation de la MS. Les géno-types transgéniques expriment le gène Lc à différents degrés quand on les cultive sous un vif éclairage, ce qui entraîne une accumulation d'anthocyanidine pouvant aller jusqu'à 136 µg par gramme de MS. Les génotypes Lc se caractérisent par une disparition véritable de la MS plus lente que la luzerne NM après 0, 4 et 12 h d'incubation, mais pas après 24 ou 48 h. Comparativement à la luzerne NM, les génotypes transgéniques Lc renferment moins de MS soluble rapidement, mais autant de MS dégradable lentement, le taux de dégradation de cette dernière étant analogue. Le décalage de la digestion est lui aussi similaire. L'azote disparaît plus lentement chez les lignées transgéniques Lc que chez la luzerne NM au début de l'incubation. La solubilité de la MS et du N présentent une corrélation négative avec la...
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