Optogenetics affords the ability to stimulate genetically targeted neurons in a relatively innocuous manner. Reliable and targetable tools have enabled versatile new classes of investigation in the study of neural systems. However, current hardware systems are generally limited to acute measurements or require external tethering of the system to the light source.Here we provide a low-cost, high-power, remotely controlled blue laser diode (LD) stimulator for the application of optogenetics in neuroscience, focusing on wearable and intelligent devices, which can be carried by monkeys, rats and any other animals under study. Compared with the conventional light emitting diode (LED) device, this LD stimulator has higher efficiency, output power, and stability. Our system is fully wirelessly controlled and suitable for experiments with a large number of animals.

The aim of this study was to clone the mRNA sequence of the ACADL gene of goats and explore the effect of ACADL on the differentiation of subcutaneous fat cells on this basis. Methods:We obtained the ACADL gene of goats by cloning and used -qPCR to detect the ACADL expression patterns of different goat tissues and subcutaneous fat cells at different lipid induction stages. In addition, we transfect intramuscular and subcutaneous adipocytes separately by constructing overexpressed ACADL vectors and synthesizing Si-ACADL; Finally, we observed the changes in oil red stained cell levels under the microscope, and qPCR detected changes in mRNA levels. Results:The results showed goat ACADL gene expressed in sebum fat. During adipocyte differentiation, ACADL gradually increased from 0 to 24 h of culture, and decreased. Overexpression of ACADL promoted differentiation of subcutaneous adipocytes in goat and inhibited their differentiation after interference. Conclusion:So, we infer ACADL may have an important role in positive regulating the differentiation process in goat subcutaneous adipocyte. This study will provide basic data for further study of the role of ACADL in goat subcutaneous adipocyte differentiation and lays the foundatin for final elucidating its molecular mechanisms in regulating subcutaneous fat deposition in goats.