Objective This research aims to analyze the impact of the novel coronavirus pandemic on the hospital visits of patients with acute appendicitis. Methods The retrospective analysis was designed to look at the treatment of acute appendicitis in the Department of General Surgery in Beijing Jishuitan Hospital before and during the COVID-19 pandemic (2019–2020). Data was analyzed by the numbers of patients, sex, age, onset time, fever or not, laboratory examination, imaging test, and treatment. And we analyzed the differences between the “pre-COVID group” and “during-COVID group”. Results Compared with the year 2019, the number of acute appendicitis patients has diminished substantially during the COVID-19 pandemic (2020), but the number elevated with the control of the pandemic. Even if we did not find the differences of the treatment before and during the pandemic (P = 0.932), the onset time to emergency was significantly longer (P < 0.001), and more patients had showed fever (P < 0.001) during the COVID-19 pandemic. And the total number of white blood cells and C reactive protein level were significantly higher in 2020 than those in 2019 (P = 0.006, 0.003). And the same result was found in patients with appendiceal fecalith (P = 0.047). Conclusion During the pandemic of the new coronavirus pneumonia, the number of patients with acute appendix treatment dropped significantly, mainly because it took longer than before, and the condition was more severe. It can be seen that the new coronary pneumonia has a great impact on the patients’ medical treatment behavior, and the active prevention and treatment of the new coronavirus pneumonia is currently an important and urgent issue.

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Comparison on the genomic structure and phylogenetic relationship of the Hsp88 genes from P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa was described. The Hsp88 genes from the three entomopathogenic strains, P. tenuipes Jocheon-1(strain), P. tenuipes(original species), and C. militaris contain the identical genomic structure, namely 5 introns and 6 exons with the length of 13, 62, 32, 1,438, 306, 288 nucleotides encoding 713 amino acid residues, whereas in case of C. pruinosa, it contains 4 introns and 5 exons with the length of 13, 62, 32, 1,744, 288 nucleotides encoding 713 amino acid residues. The genomic DNA length of the Hsp88 genes from P. tenuipes Jocheon-1 and P. tenuipes are both 2,600 nucleotides long in size. The Hsp88 genes from C. militaris and C. pruinosa are 2,582, 2,576 nucleotides long in size, respectively. Hsp88 genes of the P. tenuipes Jochoen-1, P. tenuipes, C. militaris and C. pruinosa also contain the conserved ATP-binding domain. Phylogenetic analysis of the Hsp genes of the four strains tested in this study showed that the fungal Hsp88 is divided into two separate clades, ascomycetes and deutromycete. Within the ascomycetes fungal clade, the P. tenuipes Jochoen-1 and P. tenuipes formed a subgroup, on the other hand, C. militaris and C. pruinosa formed another subgroup. Pair-wise comparison of P. tenuipes Jocheon-1 Hsp88 with those of P. tenuipes, C. militaris and C. pruinosa Hsp88s revealed significant identity in deduced amino acid sequence among these strains. The P. tenuipes Jocheon-1 Hsp88 showed 99% identity with the P. tenuipes, 97% identity with the C. militaris, and 98% identity with the C. pruinosa.

Objective: The dysregulation of circadian rhythms is proved to be associated with the development of and may affect the treatment of tumors. However, the biological role of NR1D1/2 remains elusive in gastric cancer. We analyzed the effects of NR1D1/2 expression with occurrence and prognostic value for gastric cancer. Methods: By using the online databases including ULCAN, KM plotter, STRING database and FunRich software, the effects of NR1D1/2 on the occurrence, clinical features and prognosis of gastric cancer were evaluated in gastric cancer. Results: Based on the analyses of data obtained from online database, both the mRNA expression of NR1D1 and NR1D2 were higher in stomach carcinoma than in normal tissue (p<0.001). The level of NR1D1 expression was significantly increased in female patients, T, N, and Lauren classification in gastric cancer patients (p=0.017, 0.000, 0.014, 0.005, and 0.004), and the NR1D2 was significantly higher in male patients, HER2 status, tumor stage, T, Lauren classification, and differentiation in gastric cancer patients (p=0.000, 0.000, 0.000, 0.001, 0.000 and 0.000). The elevation of NR1D1/2 was significantly correlated with worse OS (overall survival), PPS (post-progression survival) and FP (First progression) in gastric cancer patients (p<0.001). Furthermore, we constructed protein-protein interaction networks of genes coexpressed with NR1D1 and NR1D2. FunRich analyses suggested that the NR1D1 and NR1D2 was possibly involved in BMAL1: CLOCK/NPAS2 activates gene expression and circadian clock pathway. Conclusion: Our study suggests that NR1D1 and NR1D2 may be potential biomarkers for the diagnosis and prognosis of gastric cancer.

ObjectiveThe main objective was to investigate the early assessment value of Body Mass Index in the prediction of severity in acute pancreatitis. MethodsThe retrospective analysis was designed to assess the relationship between Body Mass Index and the severity of acute pancreatitis in Beijing Jishuitan Hospital from January 2018 to December 2019. Using SPSS 20.0 software for statistical analysis, and Logistic Regression for the factors affecting the severity of acute pancreatitis, and draw the ROC curve to test the effectiveness to the diagnosis of acute pancreatitis. ResultsThe BMI was significantly correlated with the Ranson scoring and MCTSI scoring (P=0.000, 0.000). The difference of BMI in MAP and SAP patients was statistically significantly (P=0.000). The Logistic Regression indicated that BMI was an independent risk factor for the severity of acute pancreatitis. Combined detection of BMI, WBC, serum calcium, and SAMY in prognosis of the severity of acute pancreatitis positive rate is higher than single positive rate. ConclusionThe BMI gradually increased with the severity of acute pancreatitis, and was an independent risk factor for the condition. Combined detection of BMI, WBC, serum calcium, and SAMY can effectively improve the acute pancreatitis patients’ clinical diagnostic accuracy and early treatment, and help to reduce the complications.