Micro-dissection technology possesses revolutionary significance in the research field about Cancer Genomics, through which pure population of targeted cells can be procured from tissue sections for subsequent analysis. In this paper, a novel Micro-dissection technology using ultrasonic vibration was proposed and the piezo-powered micro-dissection tool with its control and drive system were also developed. The micro-dissection tool employs the multilayer piezoelectric actuator for generating the ultrasonic vibration with high frequency and low amplitude. To control the piezo-powered Micro-dissection tool, a driving power for piezoelectric actuator was designed using direct digital synthesizer and direct current amplifying principle, thus the micro-dissection tool could vibrate with the frequency scope from 0.5k to50K and the amplitude scope from 0 to 2μm. Finally, experiments about bio-microdissection on liver tissue slice were carried out with the tool and better micro-dissection result was obtained. The experiments show the feasibility of the micro-dissection method with ultrasonic vibration. The new micro-dissection method can be adopted in the bio-micro-manipulation field and it can also significantly promote the development of Cancer Genomics.

Considering the long-term, flexible blood glucose monitoring demand, a sensor monitoring system for interstitial fluid (ISF) ultrafiltration sampling and on-line monitoring of blood glucose is presented. In this paper, the glucose sensor chip used in this system will be introduced in detailed. This sensor chip was developed by MEMS technique and it has the advantages of less ISF consumption, smaller structures and easier integration. Otherwise, the silicon glucose sensor chip is provided with diffusion control of the analyte through a porous silicon membrane into a silicon etched cavity containing the agarose immobilised enzyme. First, glucose monitoring principle of this sensor system will be intrudouced briefly. Then, this paper focuses on the design and fabrication of the key component, which is the MEMS sensor chip with diffusion control. Finally, experiments were carried out, and results show that the sensor chips signal response time at increasing glucose concentrations is about 5s; The linear range is large enough to cover the required broad area of blood glucose (0.2 ~ 20 mmol / L), the sensitivity is 9.76 nA/mmol.L-1, and the correlation coefficient is 0.9954. In addition, experiments results of sensor chip with different pore membrane were compared with each other. We can see that different measuring range and sensitivity can be obtained, which agrees with the theoretical analysis.

This paper introduces American shale gas resource development experience and technique. The author also discusses China's shale gas resource potential and current states of exploration and development. Based on this information, this paper puts forward some strategic concepts of accelerating shale gas development in China.

Iron-based amorphous foils and metallic aluminum foils are stacked in alternating layer and welding as laminate composites by ultrasound consolidation process with high-frequency acoustic vibrations. It makes the material to play their own advantages as the new focus. In the consolidation process, the most important standard parameters of the mechanical properties of the material are mainly controlled by the normal force, amplitude of the ultrasonic vibration and the travel speed of the sonotrode imposed by the forming. Ultrasonic vibrations, typically 20 kHz, are applied to the foil laterally (along its width) through a sonotrode (welding tool) .In this paper, the optimum parameters of 25μm amplitude, 2.5kN static force and 20mm / s traveling speed are obtained. It is well-known that the microstructure is the most intuitive way to measure the mechanical properties of materials, including hardness and so on. In addition, in this paper, the forming mechanism of Al 1080/ amorphous (Fe-Si-B) and the connection performance of ultrasonic bonding technology are described in detail. scanning electron microscopy (SEM) found that there is a mechanical interface mechanism and metallurgical bonding mechanism. And the recrystallization temperature of aluminum was calculated. The kinetics of interfacial growth at the grain boundary was analyzed. The composition of interfacial material was analyzed by energy dispersive spectroscopy (EDS) in the metallurgical bonding interface of the recrystallized region. It was preliminarily concluded that the Al-rich FeAl3 phase and the AlB2 phase were inferred. In the exfoliation experiment, it was found by X-ray diffractometry (XRD) that the amorphous did not appear to be crystallized. Finally, the laminated materials were tested for mechanical properties.

Design, simulation, flow characteristics of micro check valves used in Interstitial Fluid (ISF) devices are presented in this paper. The micro check valve includes three layers: the upper valve seat, the lower valve seat and the middle membrane layer which is fabricated by a polymeric surface micro machining process, which utilizes SU-8 as the functional material. The structure of membrane is designed as a disc with 1.2 mm diameter suspended on 4 folded beams, which work as valve springs. Maximum deformation and stress of membrane (50 μm, 100 μm, 150 μm, 200 μm, and 250 μm thicknesses) have been analyzed with ANSYS under 70 kPa. Simulation results reveal the membrane size is how to affect the performance of it. Finally, the membrane is fabricated with 150 μm SU-8 layer, and it is successfully integrated into the ISF sampling device. The forward flow and cut-off performance of the micro check valves have been observed with water. The flow curves were obtained under different forward and backward pressure. Experiment results shown that flow rates in the forward direction is small, and the flow rate is about 450 μL/min under 70 kPa; the valves can withstand more than 83 kPa pressure, and the reverse flow is nearly to zero, which meets the requirements of the ISF sampling device well.